Protein-tyrosine phosphatases PTP1B and syp are modulators of insulin-stimulated translocation of GLUT4 in transfected rat adipose cells
| Autor: | Stanley Wertheimer, Paul Burn, Chung H. Lin, Michael J. Quon, Kurt Amrein, Hui Chen, Susan L. Katz |
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| Rok vydání: | 1997 |
| Předmět: |
Male
medicine.medical_specialty Monosaccharide Transport Proteins medicine.medical_treatment Cell Adipose tissue Muscle Proteins Protein Tyrosine Phosphatase Non-Receptor Type 11 Protein tyrosine phosphatase Biology Transfection Biochemistry Internal medicine Insulin receptor substrate medicine Adipocytes Animals Insulin Molecular Biology Cells Cultured Glucose Transporter Type 4 Protein Tyrosine Phosphatase Non-Receptor Type 6 Cell Membrane Glucose transporter Intracellular Signaling Peptides and Proteins Biological Transport Cell Biology Recombinant Proteins Rats medicine.anatomical_structure Endocrinology biology.protein Protein Tyrosine Phosphatases hormones hormone substitutes and hormone antagonists GLUT4 |
| Zdroj: | The Journal of biological chemistry. 272(12) |
| ISSN: | 0021-9258 |
| Popis: | The protein-tyrosine phosphatases PTP1B and Syp have both been implicated as modulators of the mitogenic actions of insulin. However, the roles of these protein-tyrosine phosphatases in the metabolic actions of insulin are not well characterized. In this study, we directly assessed the ability of PTP1B and Syp to modulate insulin-stimulated translocation of the insulin-responsive glucose transporter GLUT4 in a physiologically relevant insulin target cell. Primary cultures of rat adipose cells were transiently transfected with either wild-type PTP1B (PTP1B-WT), wild-type Syp (Syp-WT), or the catalytically inactive mutants PTP1B-C/S or Syp-C/S. The effects of overexpression of these constructs on insulin-stimulated translocation of a co-transfected epitope-tagged GLUT4 were studied. Cells overexpressing either PTP1B-C/S or Syp-WT had insulin dose-response curves similar to those obtained with control cells expressing only epitope-tagged GLUT4. In contrast, for cells overexpressing PTP1B-WT the level of GLUT4 on the cell surface at each insulin dose (ranging from 0 to 60 nM) was significantly lower than that observed in the control cells. Interestingly, cells overexpressing the dominant inhibitory mutant Syp-C/S also had a small but statistically significant impairment in insulin responsiveness. At a maximally stimulating concentration of insulin (60 nM), cell surface epitope-tagged GLUT4 was approximately 20% less than that of the control cells. It is possible that effects from high level overexpression of Syp and PTP1B constructs may not reflect what occurs under physiological conditions. Nevertheless, our data raise the possibility that PTP1B may be a negative regulator of insulin-stimulated glucose transport, while Syp may have a small role as a positive mediator of the metabolic actions of insulin. |
| Databáze: | OpenAIRE |
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