Counting circulating endothelial cells in allo-HSCT: an ad hoc designed polychromatic flowcytometry-based panel versus the CellSearch System
Autor: | Gianluca Rotta, Kurt Baeten, Andrea Bianchetti, Domenico Russo, Mirella Marini, Simona Braga, Paola Omedè, Benedetto Bruno, Valeria Cancelli, Andrea Di Palma, Arabella Neva, Rosanna Verardi, Giovanna Piovani, Cristina Skert, Camillo Almici |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Oncology medicine.medical_specialty medicine.medical_treatment Allo hsct Graft vs Host Disease lcsh:Medicine Hematopoietic stem cell transplantation Article 03 medical and health sciences 0302 clinical medicine Hematologic disorders Internal medicine medicine Humans Transplantation Homologous Bland–Altman plot lcsh:Science Blood Cells Multidisciplinary business.industry lcsh:R Limits of agreement Hematopoietic Stem Cell Transplantation Endothelial Cells Flow Cytometry Clinical routine Transplantation surgical procedures operative 030104 developmental biology lcsh:Q business 030217 neurology & neurosurgery |
Zdroj: | Scientific Reports, Vol 9, Iss 1, Pp 1-12 (2019) Scientific Reports |
Popis: | Physio-pathologic interrelationships between endothelial layer and graft-versus-host disease (GVHD) have been described leading to assess the entity “endothelial GVHD” as the early step for clinical manifestations of acute GVHD. The availability of the CellSearch system has allowed us to monitor Circulating Endothelial Cells (CEC) changes in allogeneic hematopoietic stem cell transplantation (allo-HSCT) as useful tool to help clinicians in GVHD diagnostic definition. We have compared CEC counts generated by an ad hoc designed polychromatic-flowcytometry (PFC) Lyotube with those of the CellSearch system. CEC were counted in parallel at 5 timepoints in 50 patients with malignant hematologic disorders undergoing allo-HSCT (ClinicalTrials.gov, NCT02064972). Spearman rank correlation showed significant association between CEC values at all time points (p = 0.0001). The limits of agreement was demonstrated by Bland Altman plot analysis, showing bias not significant at T1, T3, T4, while at T2 and T5 resulted not estimable. Moreover, Passing Bablok regression analysis showed not significant differences between BD Lyotube and CellSearch system. We show that CEC counts, generated with either the CellSearch system or the PFC-based panel, have a superimposable kinetic in allo-HSCT patients and that both counting procedures hold the potential to enter clinical routine as a suitable tool to assist clinicians in GVHD diagnosis. |
Databáze: | OpenAIRE |
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