Development of a lavage procedure to collect lung secretions from chickens for evaluating respiratory humoral immunity
| Autor: | Peter S. Holt, Richard K. Gast, R. W. Moore, Henry D. Stone |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Salmonella Infections
Animal Lung General Immunology and Microbiology Salmonella enteritidis Antibody level respiratory system Biology Antibodies Bacterial Bronchoalveolar Lavage Sampling system medicine.anatomical_structure Food Animals Mucosal immunology Humoral immunity Immunology Antibody Formation medicine Animals Animal Science and Zoology Respiratory system Chickens Syringe Poultry Diseases |
| Zdroj: | Avian pathology : journal of the W.V.P.A. 34(5) |
| ISSN: | 0307-9457 |
| Popis: | Mucosal immunology research has been hampered by the difficulty and labour-intensiveness of collecting samples. This is especially true for sites such as the lung, and the present paper describes a simple method for obtaining samples from this organ in chickens. Following sacrifice, the bird was placed on its back and the trachea was cut and exteriorized. Narrow-diameter tubing, to which a 30 ml syringe was attached, was threaded down the trachea to the bronchi and air was evacuated from the lung. Warm buffer was administered and the lung sample then aspirated, processed and frozen. In the current experiment this sampling system was tested on hens that were challenged with Salmonella Enteritidis. Elevated anti-Salmonella Enteritidis antibody levels in lung from infected hens were observed in significantly more infected hens than non-infected control hens in two trials. The simplicity and utility of this sampling system will make it a useful tool for those laboratories wishing to expand their humoral mucosal immunology capabilities, even for study of non-respiratory pathogens. |
| Databáze: | OpenAIRE |
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