Mitogen-activated protein kinase phosphatase-1 in rat arterial smooth muscle cell proliferation
| Autor: | Mu-En Lee, Hong Wang, Mukesh K. Jain, Wen Sen Lee, Edgar Haber, Kaihua Lai |
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| Rok vydání: | 1996 |
| Předmět: |
Male
Mitogen-Activated Protein Kinase 3 Cell Cycle Proteins Protein tyrosine phosphatase Pulmonary Artery Muscle Development Gene Expression Regulation Enzymologic Muscle Smooth Vascular Immediate-Early Proteins Organ Culture Techniques Protein Phosphatase 1 Phosphoprotein Phosphatases Animals ASK1 Tissue Distribution Phosphorylation MAPK14 biology Kinase Smooth muscle layer Cell Cycle Protein phosphatase 1 Dual Specificity Phosphatase 1 General Medicine Arteries Recombinant Proteins Cell biology Rats Carotid Arteries Mitogen-activated protein kinase Calcium-Calmodulin-Dependent Protein Kinases biology.protein Mitogen-Activated Protein Kinases Protein Tyrosine Phosphatases Carotid Artery Injuries Angioplasty Balloon Research Article |
| Zdroj: | The Journal of clinical investigation. 98(7) |
| ISSN: | 0021-9738 |
| Popis: | Smooth muscle cell proliferation and migration is important in arteriosclerosis. In this process, cytokines and growth factors are upregulated and bind to their respective receptors, which in turn stimulate mitogen-activated protein (MAP) kinases. MAP kinases then relay signals to the nucleus that activate quiescent smooth muscle cells. Phosphatases downregulate MAP kinases. We investigated the role of a dual-specificity tyrosine phosphatase, MAP kinase phosphatase-1 (MKP-1), in smooth muscle cell proliferation. MKP-1 expression was high in arterial tissue by Northern analysis, and MKP-1 message was detected mainly in the arterial smooth muscle layer by in situ hybridization. After balloon injury of the rat carotid artery, expression of MKP-1 decreased greatly, whereas that of MAP kinases, especially p44 MAP kinase, increased. The time course of the reduction in MKP-1 message correlated with increased tyrosine phosphorylation and elevated p44 MAP kinase enzymatic activity. In rat arterial smooth muscle cells overexpressing MKP-1, growth was arrested in the G1 phase and entry into the S phase was blocked. A reduction in MKP-1 expression may contribute in part to proliferation of smooth muscle cells after vascular injury, possibly through a decrease in dephosphorylation of p44 MAP kinase. |
| Databáze: | OpenAIRE |
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