Activity of Antioxidants from Crocus sativus L. Petals: Potential Preventive Effects towards Cardiovascular System
| Autor: | Maria Adelaide Continenza, Alberto Chiarini, Randolph R.J. Arroo, Pasquale Marrazzo, Matteo Micucci, Keti Zeka, Cristina Angeloni, Silvana Hrelia, Guido Macchiarelli, Stefania A. Nottola, Roberta Budriesi, Ketan C. Ruparelia |
|---|---|
| Přispěvatelé: | Zeka K, Marrazzo P, Micucci M, Ruparelia KC, Arroo RRJ, Macchiarelli G, Nottola SA, Continenza MA, Chiarini A, Angeloni C, Hrelia S, Budriesi R., Zeka, Keti [0000-0002-3727-9522], Marrazzo, Pasquale [0000-0001-6644-5828], Micucci, Matteo [0000-0003-3525-1531], Arroo, Randolph R. J. [0000-0002-0672-7927], Macchiarelli, Guido [0000-0002-9182-0586], Angeloni, Cristina [0000-0002-6731-6187], Hrelia, Silvana [0000-0001-7857-4512], Budriesi, Roberta [0000-0002-8454-9740], Apollo - University of Cambridge Repository, Arroo, Randolph RJ [0000-0002-0672-7927] |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Chronotropic Antioxidant Physiology medicine.medical_treatment Clinical Biochemistry ved/biology.organism_classification_rank.species Pharmacology Biochemistry Article Crocin 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine nutraceuticals Crocus sativus L. petals Crocus sativus by-product medicine MTT assay Viability assay Molecular Biology by-products Crocus kaempferol biology Chemistry ved/biology lcsh:RM1-950 cardiovascular prevention Cell Biology biology.organism_classification crocin Crocus sativus L. petal lcsh:Therapeutics. Pharmacology 030104 developmental biology By-products Cardiovascular prevention Kaempferol Nutraceuticals 030220 oncology & carcinogenesis |
| Zdroj: | Antioxidants Volume 9 Issue 11 Antioxidants, Vol 9, Iss 1102, p 1102 (2020) |
| Popis: | The petals of the saffron crocus (Crocus sativus L.) are considered a waste material in saffron production, but may be a sustainable source of natural biologically active substances of nutraceutical interest. The aim of this work was to study the cardiovascular effects of kaempferol and crocin extracted from saffron petals. The antiarrhythmic, inotropic, and chronotropic effects of saffron petal extract (SPE), kaempferol, and crocin were evaluated through in vitro biological assays. The antioxidant activity of kaempferol and crocin was investigated through the 2&prime 7&prime dichlorodihydrofluorescein diacetate (DCFH-DA) assay using rat cardiomyoblast cell line H9c2. The MTT assay was applied to assess the effects of kaempferol and crocin on cell viability. SPE showed weak negative inotropic and chronotropic intrinsic activities but a significant intrinsic activity on smooth muscle with a potency on the ileum greater than on the aorta: EC50 = 0.66 mg/mL versus EC50 = 1.45 mg/mL. Kaempferol and crocin showed a selective negative inotropic activity. In addition, kaempferol decreased the contraction induced by KCl (80 mM) in guinea pig aortic and ileal strips, while crocin had no effect. Furthermore, following oxidative stress, both crocin and kaempferol decreased intracellular ROS formation and increased cell viability in a concentration-dependent manner. The results indicate that SPE, a by-product of saffron cultivation, may represent a good source of phytochemicals with a potential application in the prevention of cardiovascular diseases. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|