Molecular diversity and distribution of marine fungi across 130 European environmental samples
| Autor: | Finlay Maguire, Meredith D. M. Jones, Frédéric Mahé, Micah Dunthorn, Colomban de Vargas, Aurélie Chambouvet, Thomas A. Richards, Guy Leonard, Ramon Massana, Javier del Campo, Sarah Romac |
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| Přispěvatelé: | Department of ecology, Technische Universität Kaiserslautern (TU Kaiserslautern), Instituto de Ciencia de Materiales de Aragón [Saragoza, España] (ICMA-CSIC), University of Zaragoza - Universidad de Zaragoza [Zaragoza], Evolution des Protistes et Ecosystèmes Pélagiques (EPEP), Adaptation et diversité en milieu marin (AD2M), Station biologique de Roscoff [Roscoff] (SBR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff [Roscoff] (SBR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Life Sciences London, The Natural History Museum [London] (NHM), Universität Kaiserslautern, Institute of Marine Sciences / Institut de Ciències del Mar [Barcelona] (ICM), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), University of Exeter |
| Rok vydání: | 2015 |
| Předmět: |
Geologic Sediments
[SDV]Life Sciences [q-bio] Molecular Sequence Data Biology General Biochemistry Genetics and Molecular Biology Dikarya sediment communities 03 medical and health sciences Phylogenetics Abundance (ecology) Chytrids Environmental DNA Seawater 14. Life underwater DNA Fungal Relative species abundance Marine fungi Research Articles Phylogeny 030304 developmental biology General Environmental Science 0303 health sciences General Immunology and Microbiology Phylogenetic tree 030306 microbiology Ecology Microbiota Fungi Genetic Variation RNA Fungal General Medicine Sequence Analysis DNA 15. Life on land biology.organism_classification chytrids Europe Microscopy Fluorescence Evolutionary biology [SDE]Environmental Sciences Pyrosequencing 454 pyrosequencing General Agricultural and Biological Sciences Sediment communities |
| Zdroj: | Proceedings Of The Royal Society B-biological Sciences (0962-8452) (Royal Soc), 2015-11, Vol. 282, N. 1819, P. 1-10 Proceedings of the Royal Society B: Biological Sciences Proceedings of the Royal Society B: Biological Sciences, 2015, 282 (1819), pp.20152243. ⟨10.1098/rspb.2015.2243⟩ Proceedings of the Royal Society B: Biological Sciences, Royal Society, The, 2015, 282 (1819), pp.20152243. ⟨10.1098/rspb.2015.2243⟩ Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0962-8452 1471-2954 |
| Popis: | Richards, Thomas A. ... et. al.-- 10 pages, 5 figures, 1 table, electronic supplementary material https://royalsocietypublishing.org/doi/suppl/10.1098/rspb.2015.2243 Environmental DNA and culture-based analyses have suggested that fungi are present in low diversity and in low abundance in many marine environments, especially in the upper water column. Here, we use a dual approach involving high-throughput diversity tag sequencing from both DNA and RNA templates and fluorescent cell counts to evaluate the diversity and relative abundance of fungi across marine samples taken from six European near-shore sites. We removed very rare fungal operational taxonomic units (OTUs) selecting only OTUs recovered from multiple samples for a detailed analysis. This approach identified a set of 71 fungal ‘OTU clusters' that account for 66% of all the sequences assigned to the Fungi. Phylogenetic analyses demonstrated that this diversity includes a significant number of chytrid-like lineages that had not been previously described, indicating that the marine environment encompasses a number of zoosporic fungi that are new to taxonomic inventories. Using the sequence datasets, we identified cases where fungal OTUs were sampled across multiple geographical sites and between different sampling depths. This was especially clear in one relatively abundant and diverse phylogroup tentatively named Novel Chytrid-Like-Clade 1 (NCLC1). For comparison, a subset of the water column samples was also investigated using fluorescent microscopy to examine the abundance of eukaryotes with chitin cell walls. Comparisons of relative abundance of RNA-derived fungal tag sequences and chitin cell-wall counts demonstrate that fungi constitute a low fraction of the eukaryotic community in these water column samples. Taken together, these results demonstrate the phylogenetic position and environmental distribution of 71 lineages, improving our understanding of the diversity and abundance of fungi in marine environments The work is part of the BiodivERsA ERA-Net programme BioMarKs (Biodiversity of Marine euKaryotes). T.A.R. is supported by the Gordon and Betty Moore Foundation (grant no. GBMF3307). Fr.M. and M.D. are supported by a grant from the Deutsche Forschungsgemeinschaft (DFG) DU1319/1–1. J.d.C. is supported by a Marie Curie International Outgoing Fellowship (FP7-PEOPLE-2012-IOF, 331450-CAARL). A.C. is supported by a Marie Curie Intra-European Fellowship (FP7-PEOPLE-2011-IEF, 299815-PARAFROGS) and a EMBO Long-Term fellowship (ATL-1069-2011) |
| Databáze: | OpenAIRE |
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