Prep1 prevents premature adipogenesis of mesenchymal progenitors
| Autor: | Raffaele Luongo, Dmitry Penkov, Marco J. Morelli, Maria Cristina Magli, Elena Levantini, Giorgia Maroni, Francesco Blasi, Alexander V. Egorov, Vsevolod A. Tkachuk |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Repressor lcsh:Medicine Bone Marrow Cells Article 03 medical and health sciences chemistry.chemical_compound Mice Adipocyte 3T3-L1 Cells Adipocytes Animals Humans transcriptional regulation Enhancer lcsh:Science Transcription factor Regulation of gene expression Homeodomain Proteins Multidisciplinary Adipogenesis Ccaat-enhancer-binding proteins CCAAT-Enhancer-Binding Protein-beta Mesenchymal stem cell lcsh:R Gene Expression Regulation Developmental Cell Differentiation Mesenchymal Stem Cells Cell biology hypomorphic mice ChIP-seq DNA-Binding Proteins 030104 developmental biology Enhancer Elements Genetic chemistry transgenic model lcsh:Q bone-marrow mesenchymal stem cells RNA-seq adipogenic differentiation C/EBP beta binding to chromatin |
| Zdroj: | Scientific Reports Scientific reports (Nature Publishing Group) 7 (2017). doi:10.1038/s41598-017-15828-1 info:cnr-pdr/source/autori:Maroni, Giorgia; Tkachuk, Vsevolod A.; Egorov, Alexander; Morelli, Marco J.; Luongo, Raffaele; Levantini, Elena; Blasi, Francesco; Magli, Maria Cristina; Penkov, Dmitry/titolo:Prep1 prevents premature adipogenesis of mesenchymal progenitors/doi:10.1038%2Fs41598-017-15828-1/rivista:Scientific reports (Nature Publishing Group)/anno:2017/pagina_da:/pagina_a:/intervallo_pagine:/volume:7 Scientific Reports, Vol 7, Iss 1, Pp 1-10 (2017) |
| ISSN: | 2045-2322 |
| Popis: | Transcriptional regulators are crucial in adipocyte differentiation. We now show that the homeodomain-containing transcription factor Prep1 is a repressor of adipogenic differentiation since its down-regulation (DR) in both ex vivo bone marrow-derived mesenchymal stromal cells (MSC) and in vitro 3T3-L1 preadipocytes significantly increases their adipogenic differentiation ability. Prep1 acts at a stage preceding the activation of the differentiation machinery because its DR makes cells more prone to adipogenic differentiation even in the absence of the adipogenic inducers. Prep1 DR expands the DNA binding landscape of C/EBPβ (CCAAT enhancer binding protein β) without affecting its expression or activation. The data indicate that Prep1 normally acts by restricting DNA binding of transcription factors to adipogenic enhancers, in particular C/EBPβ. |
| Databáze: | OpenAIRE |
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