The expression of leptin and its receptors in pre-ovulatory human follicles

Autor: H R Snodgrass, Shafer Aw, M Antczak, J A Cioffi, J Van Blerkom, S Wittmer
Rok vydání: 1997
Předmět:
Adult
Leptin
endocrine system
Embryology
medicine.medical_specialty
media_common.quotation_subject
Gene Expression
Receptors
Cell Surface
Fertilization in Vitro
Biology
In Vitro Techniques
Polymerase Chain Reaction
Ovarian Follicle
Pregnancy
Internal medicine
Follicular phase
Genetics
medicine
Humans
Ovarian follicle
Receptor
Molecular Biology
Ovulation
media_common
DNA Primers
Granulosa Cells
Base Sequence
urogenital system
digestive
oral
and skin physiology
Embryogenesis
Obstetrics and Gynecology
Proteins
Cell Biology
Hair follicle
Embryo Transfer
Follicular fluid
Follicular Fluid
Endocrinology
medicine.anatomical_structure
Reproductive Medicine
Follicular Phase
Infertility
Oocytes
Receptors
Leptin
Female
Carrier Proteins
hormones
hormone substitutes
and hormone antagonists
Developmental Biology
Zdroj: Scopus-Elsevier
ISSN: 1360-9947
Popis: The expression of leptin and its receptors was examined by reverse transcriptase-polymerase chain reaction and immunofluorescence in granulosa and cumulus cells of pre-ovulatory follicles and in meiotically mature oocytes obtained from women undergoing in-vitro fertilization. Leptin concentrations were measured in newly aspirated follicular fluids and in maternal serum before and after the administration of an ovulatory dose of human chorionic gonadotrophin. The findings demonstrate leptin expression at the mRNA and protein levels by granulosa and cumulus cells, and the presence of leptin in mature human oocytes. While an association between follicular leptin concentration and embryo development was not observed, a post-ovulatory increase in serum leptin concentration was associated with implantation potential. The results are discussed with respect to possible roles of leptin in early human development.
Databáze: OpenAIRE
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