Real-time detection of mitochondrial inhibition at frog motor nerve terminals using increases in the spatial variance in probability of transmitter release
| Autor: | Michael D. Miyamoto, Spencer D. Provan |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Motor Neurons
Neurotransmitter Agents Chemistry General Neuroscience Rana pipiens Spatial Behavior Motor nerve Mercury Mitochondrion Flufenamic Acid Mitochondria Rana Electrophysiology Flufenamic acid Potassium Biophysics medicine Animals GRENOUILLE Neurosecretion Neuroscience Free nerve ending medicine.drug |
| Zdroj: | Neuroscience Letters. 185:187-190 |
| ISSN: | 0304-3940 |
| DOI: | 10.1016/0304-3940(95)91365-o |
| Popis: | The effects of Hg 2+ , methyl mercury, and flufenamic acid, all of which inhibit mitochondria, were examined at frog motor nerve terminals. Unbiased estimates of m (no. of transmitter quanta released), n (no. of functional release sites), p (probability of release), and var s p (spatial variance in p ) were obtained using K + -induced asynchronous neurosecretion ( m , n and p not having the same definitions as with nerve-evoked release). Transient but significant increases in m , n , p and var s p were found with all three agents. These findings indicate that mitochondrial inhibition and release of sequestered Ca 2+ can be detected as a real-time increase in var s p . The results also suggest that changes in var s p might be used to differentiate between cellular (membrane) and subcellular (organellar) actions of drugs at the nerve terminal. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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