Do heat shock proteins play a role in Graves' disease? Heat shock protein-specific T-cells from Graves' disease thyroids do not recognize thyroid epithelial cells

Autor: J Szabo, A Sztankay, Trieb K, M Hermann, Gratzl R, B Grubeck-Loebenstein, S Jindal
Rok vydání: 1993
Předmět:
Male
Chaperonins
T-Lymphocytes
Endocrinology
Diabetes and Metabolism
Graves' disease
Clinical Biochemistry
Thyroid Gland
Fluorescent Antibody Technique
Lymphocyte Activation
medicine.disease_cause
Biochemistry
Epithelium
Autoimmunity
law.invention
Endocrinology
law
Cells
Cultured
Heat-Shock Proteins
Cell Line
Transformed
Transient erythroblastopenia of childhood
Thyroid
hemic and immune systems
Middle Aged
Graves Disease
Recombinant Proteins
medicine.anatomical_structure
Recombinant DNA
Female
tissues
Adult
medicine.medical_specialty
Blotting
Western
education
Autoimmune Diseases
Cell Line
Bacterial Proteins
Heat shock protein
Internal medicine
medicine
Humans
Heat shock
Aged
Thyroid Epithelial Cells
business.industry
Biochemistry (medical)
Chaperonin 60
medicine.disease
Clone Cells
business
Zdroj: The Journal of Clinical Endocrinology & Metabolism. 77:528-535
ISSN: 1945-7197
0021-972X
DOI: 10.1210/jcem.77.2.8345059
Popis: Thyroid-derived T-cells from patients with Graves' disease were analyzed for their reactivity to recombinant heat shock proteins (hsp) and autologous thyroid epithelial cells (TEC). Five of six uncloned T-cell lines responded to stimulation with recombinant mycobacterial 71-kilodalton (kDa) hsp and cross-reacted with the corresponding amoebial and human proteins. Only one line reacted with recombinant 65-kDa hsp. Thyroid-derived T-cell lines also showed a proliferative response to TEC, which could be increased in four of the lines, when hsp expression was induced in thyroid cells by heat stress before the initiation of coculture. Clonal specificity analysis of thyroid-derived T-cell clones, however, demonstrated that distinct T-cells were responsible for the recognition of recombinant hsp and TEC. None of the clones responsive to recombinant hsp recognized TEC, whereas TEC-responsive clones did not react with recombinant hsp. Interestingly, the response of the majority of TEC-reactive clones could be dramatically increased when heat-shocked TEC were used as stimulator cells. These results suggest that T-cells specific for hsp of the 70- or 60-kDa families do not recognize TEC in the autoimmune thyroid gland. Heat shock-inducible proteins may, however, still play a role in the autoimmune process by facilitating the presentation of thyroid-specific autoantigen(s) to autoreactive T-cells.
Databáze: OpenAIRE
Externí odkaz: