Initiation, long-term cryopreservation, and recovery of Abies alba Mill. embryogenic cell lines
| Autor: | Jana Krajňáková, Dušan Gömöry, Alberto Bertolini, Angelo Vianello, Hely Häggman |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
0106 biological sciences
Somatic embryogenesis Vegetative reproduction Proliferation Germination Plant Science 01 natural sciences Cryopreservation 03 medical and health sciences chemistry.chemical_compound Abies alba Maturation Botany Abscisic acid 030304 developmental biology 0303 health sciences biology Embryo biology.organism_classification chemistry Developmental biology 010606 plant biology & botany Biotechnology |
| Zdroj: | In Vitro Cellular & Developmental Biology - Plant. 49:560-571 |
| ISSN: | 1475-2689 1054-5476 |
| DOI: | 10.1007/s11627-013-9512-1 |
| Popis: | Somatic embryogenesis of Abies alba (Mill.) has significant potential to become an effective method for vegetative propagation of this species. To induce somatic embryogenesis in A. alba, the influence of the mother tree, sampling dates, and cold treatment storage of cones were examined. The initiation frequencies ranged from 1.7% to 16.6%. The sampling date and cone storage, but not the mother tree, had a significant effect on the initiation of embryogenic cultures. Storage of embryogenic cell lines was tested through cryopreservation for 6 yr. Four out of 12 cryostored embryogenic cell lines recovered, and the regeneration of cotyledonary embryos was obtained with two cell lines. The ability of embryogenic cell masses to produce somatic embryos and the mean number of cotyledonary embryos were higher when the maturation protocol was based on embryogenic suspensions dispersed on filter paper. The properly developed germinants were obtained only from maturation media where 32 μM abscisic acid was used, being 16.2% when polyethylene glycol (PEG) was not present and 1.8% when supplemented with 10% (w/v) PEG, respectively. The present study provides evidence that it is possible to cryopreserve A. alba embryogenic cultures while maintaining their maturing ability for the lengthy period (6 yr) needed for progeny testing of field-grown trees. Therefore, our findings are important for further studies and advanced breeding work of the species; however, the conversion of germinants into ex vitro conditions still remains a significant challenge. |
| Databáze: | OpenAIRE |
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