Maintenance of sweat glands by stem cells located in the acral epithelium
| Autor: | Naho Atsumi, Yoshihiro Komai, Yoko Tokuyama, Hiroyuki Okamoto, Keiki Kumano, Shuichi Ohe, Haruyuki Ohsugi, Kiyomichi Tanaka, Shohei Kanno, Toshihiro Tanaka, Naohiro Nakamura, Fumikazu Yamazaki, Hirotsugu Yanai, Kazuhiko Ishigaki, Kazuho Saiga, Naoko Yoshida, Taichi Omachi, Hiroko Hisha, Hiroo Ueno |
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| Rok vydání: | 2015 |
| Předmět: |
Male
Cellular differentiation Biophysics Mice Transgenic Biology Biochemistry Epithelium Receptors G-Protein-Coupled Mice Imaging Three-Dimensional Proto-Oncogene Proteins Animals Cell Lineage Molecular Biology Cell Proliferation Skin Polycomb Repressive Complex 1 Skin repair Stem cell B lymphoma Mo-MLV insertion region 1 Leucine-rich repeat-containing G protein-coupled receptor 6 integumentary system Multipotent Stem Cells Stem Cells Body Weight Myoepithelial cell Cell Biology Acral epithelium Leucine-rich repeat-containing G protein-coupled receptor 5 Sweat Glands Cell biology Mice Inbred C57BL Gene Expression Regulation Multipotent Stem Cell Amniotic epithelial cells Immunology Female Epidermis Wound healing Adult stem cell |
| Zdroj: | Biochemical and Biophysical Research Communications. 466:333-338 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2015.09.022 |
| Popis: | The skin is responsible for a variety of physiological functions and is critical for wound healing and repair. Therefore, the regenerative capacity of the skin is important. However, stem cells responsible for maintaining the acral epithelium had not previously been identified. In this study, we identified the specific stem cells in the acral epithelium that participate in the long-term maintenance of sweat glands, ducts, and interadnexal epidermis and that facilitate the regeneration of these structures following injury. Lgr6-positive cells and Bmi1-positive cells were found to function as long-term multipotent stem cells that maintained the entire eccrine unit and the interadnexal epidermis. However, while Lgr6-positive cells were rapidly cycled and constantly supplied differentiated cells, Bmi1-positive cells were slow to cycle and occasionally entered the cell cycle under physiological conditions. Upon irradiation-induced injury, Bmi1-positive cells rapidly proliferated and regenerated injured epithelial tissue. Therefore, Bmi1-positive stem cells served as reservoir stem cells. Lgr5-positive cells were rapidly cycled and maintained only sweat glands; therefore, we concluded that these cells functioned as lineage-restricted progenitors. Taken together, our data demonstrated the identification of stem cells that maintained the entire acral epithelium and supported the different roles of three cellular classes. |
| Databáze: | OpenAIRE |
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