Sodium butyrate enhances intestinal integrity, inhibits mast cell activation, inflammatory mediator production and JNK signaling pathway in weaned pigs
Autor: | Fei Xie, Caihong Hu, Fang Hui Lin, Huan Wu, Jie Feng, Yan Peng, Rong Gong, Chun Chun Wang |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
MAPK/ERK pathway Swine Cell Count Growth Cell Degranulation chemistry.chemical_compound Intestinal mucosa Electric Impedance Mast Cells Intestinal Mucosa weaned pigs Barrier function biology Chemistry Sodium butyrate 04 agricultural and veterinary sciences Mast cell Intestines Infectious Diseases medicine.anatomical_structure Jejunum Biochemistry Inflammation Mediators Histamine medicine.medical_specialty MAP Kinase Signaling System p38 mitogen-activated protein kinases inflammatory mediators Immunology Tryptase Weaning Microbiology 03 medical and health sciences Internal medicine medicine Animals Molecular Biology 0402 animal and dairy science Cell Biology Original Articles 040201 dairy & animal science Diet intestinal barrier 030104 developmental biology Endocrinology mitogen-activated protein kinases Dietary Supplements biology.protein Butyric Acid mast cell |
Zdroj: | Innate Immunity |
ISSN: | 1753-4267 |
Popis: | The present study aimed to investigate the effects of sodium butyrate on the intestinal barrier and mast cell activation, as well as inflammatory mediator production, and determine whether mitogen-activated protein kinase signaling pathways are involved in these processes. A total of 72 piglets, weaned at 28 ± 1 d age, were allotted to two dietary treatments (control vs. 450 mg/kg sodium butyrate) for 2 wk. The results showed that supplemental sodium butyrate increased daily gain, improved intestinal morphology, as indicated by greater villus height and villus height:crypt depth ratio, and intestinal barrier function reflected by increased transepithelial electrical resistance and decreased paracellular flux of dextran (4 kDa). Moreover, sodium butyrate reduced the percentage of degranulated mast cells and its inflammatory mediator content (histamine, tryptase, TNF-α and IL-6) in the jejunum mucosa. Sodium butyrate also decreased the expression of mast cell-specific tryptase, TNF-α and IL-6 mRNA. Sodium butyrate significantly decreased the phosphorylated ratio of JNK whereas not affecting the phosphorylated ratios of ERK and p38. The results indicated that the protective effects of sodium butyrate on intestinal integrity were closely related to inhibition of mast cell activation and inflammatory mediator production, and that the JNK signaling pathway was likely involved in this process. |
Databáze: | OpenAIRE |
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