Sodium butyrate enhances intestinal integrity, inhibits mast cell activation, inflammatory mediator production and JNK signaling pathway in weaned pigs

Autor: Fei Xie, Caihong Hu, Fang Hui Lin, Huan Wu, Jie Feng, Yan Peng, Rong Gong, Chun Chun Wang
Rok vydání: 2017
Předmět:
0301 basic medicine
MAPK/ERK pathway
Swine
Cell Count
Growth
Cell Degranulation
chemistry.chemical_compound
Intestinal mucosa
Electric Impedance
Mast Cells
Intestinal Mucosa
weaned pigs
Barrier function
biology
Chemistry
Sodium butyrate
04 agricultural and veterinary sciences
Mast cell
Intestines
Infectious Diseases
medicine.anatomical_structure
Jejunum
Biochemistry
Inflammation Mediators
Histamine
medicine.medical_specialty
MAP Kinase Signaling System
p38 mitogen-activated protein kinases
inflammatory mediators
Immunology
Tryptase
Weaning
Microbiology
03 medical and health sciences
Internal medicine
medicine
Animals
Molecular Biology
0402 animal and dairy science
Cell Biology
Original Articles
040201 dairy & animal science
Diet
intestinal barrier
030104 developmental biology
Endocrinology
mitogen-activated protein kinases
Dietary Supplements
biology.protein
Butyric Acid
mast cell
Zdroj: Innate Immunity
ISSN: 1753-4267
Popis: The present study aimed to investigate the effects of sodium butyrate on the intestinal barrier and mast cell activation, as well as inflammatory mediator production, and determine whether mitogen-activated protein kinase signaling pathways are involved in these processes. A total of 72 piglets, weaned at 28 ± 1 d age, were allotted to two dietary treatments (control vs. 450 mg/kg sodium butyrate) for 2 wk. The results showed that supplemental sodium butyrate increased daily gain, improved intestinal morphology, as indicated by greater villus height and villus height:crypt depth ratio, and intestinal barrier function reflected by increased transepithelial electrical resistance and decreased paracellular flux of dextran (4 kDa). Moreover, sodium butyrate reduced the percentage of degranulated mast cells and its inflammatory mediator content (histamine, tryptase, TNF-α and IL-6) in the jejunum mucosa. Sodium butyrate also decreased the expression of mast cell-specific tryptase, TNF-α and IL-6 mRNA. Sodium butyrate significantly decreased the phosphorylated ratio of JNK whereas not affecting the phosphorylated ratios of ERK and p38. The results indicated that the protective effects of sodium butyrate on intestinal integrity were closely related to inhibition of mast cell activation and inflammatory mediator production, and that the JNK signaling pathway was likely involved in this process.
Databáze: OpenAIRE
Externí odkaz: