Interaction between electrical stimulation, protein coating and matrix elasticity: a complex effect on muscle fibre maturation
| Autor: | Fpt Frank Baaijens, van der Dwj Daisy Schaft, Mark J. Post, Kjm Kristel Boonen |
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| Přispěvatelé: | Fysiologie, RS: CARIM School for Cardiovascular Diseases, Soft Tissue Biomech. & Tissue Eng. |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Male
Muscle tissue Time Factors Muscle Fibers Skeletal Biomedical Engineering Muscle Proteins Medicine (miscellaneous) matrix proteins Stimulation Muscle Development muscle progenitor cells Polymerase Chain Reaction Biomaterials Extracellular matrix Mice In vivo medicine Animals Actinin Progenitor cell Elasticity (economics) electrical stimulation Cells Cultured Extracellular Matrix Proteins Chemistry maturation Cell Differentiation Myoblast maturation differentiation Elasticity Electric Stimulation In vitro Extracellular Matrix Cell biology Mice Inbred C57BL medicine.anatomical_structure Gene Expression Regulation substrate elasticity Muscle Contraction Biomedical engineering |
| Zdroj: | Journal of Tissue Engineering and Regenerative Medicine, 5(1), 60-68. Wiley |
| ISSN: | 1932-6254 |
| Popis: | In skeletal muscle tissue engineering, it remains a challenge to produce mature, functional muscle tissue. Mimicking the in vivo niche in in vitro culture might overcome this problem. Niche components include, for example, extracellular matrix proteins, neighbouring cells, growth factors and physical factors such as the elasticity of the matrix. Previously, we showed the effects of matrix stiffness and protein coating on proliferation and differentiation of muscle progenitor cells in a two-dimensional (2D) situation. In the present study we have investigated the additional effect of electrical stimulation. More precisely, we investigated the effect of electrical stimulation on primary myoblast maturation when cultured on top of Matrigel™- or laminin-coated substrates with varying elasticities. The effect of electrical stimulation on differentiation and maturation was found to be dependent on coating and stiffness. Although electrical stimulation enhanced myoblast maturation, the effect was mild. We therefore conclude that, with the current regimen, electrical stimulation is not essential to create functional, mature muscle tissue. Copyright © 2010 John Wiley & Sons, Ltd. |
| Databáze: | OpenAIRE |
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