The influence of tethered epidermal growth factor on connective tissue progenitor colony formation
| Autor: | Alan Wells, Ada Au, Cynthia Boehm, Richard Rozic, George F. Muschler, Linda G. Griffith, Nicholas A. Marcantonio |
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| Přispěvatelé: | Massachusetts Institute of Technology. Department of Biological Engineering, Massachusetts Institute of Technology. Department of Mechanical Engineering, Marcantonio, Nicholas A., Au, Ada, Griffith, Linda G. |
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Serum
Pathology medicine.medical_specialty Polymers Biophysics Connective tissue Cell Count Bioengineering Context (language use) Article Colony-Forming Units Assay Biomaterials Osteogenesis Epidermal growth factor Cell Adhesion medicine Humans Growth factor receptor inhibitor Epidermal growth factor receptor Progenitor cell Cell Size Connective Tissue Cells Epidermal Growth Factor biology Stem Cells Mesenchymal stem cell Cell Differentiation Fibronectins Cell biology medicine.anatomical_structure Solubility Mechanics of Materials Ceramics and Composites biology.protein Adsorption Stem cell Peptides |
| Zdroj: | PMC |
| Popis: | Strategies to combine aspirated marrow cells with scaffolds to treat connective tissue defects are gaining increasing clinical attention and use. In situations such as large defects where initial survival and proliferation of transplanted connective tissue progenitors (CTPs) are limiting, therapeutic outcomes might be improved by using the scaffold to deliver growth factors that promote the early stages of cell function in the graft. Signaling by the epidermal growth factor receptor (EGFR) plays a role in cell survival and has been implicated in bone development and homeostasis. Providing epidermal growth factor (EGF) in a scaffold-tethered format may sustain local delivery and shift EGFR signaling to pro-survival modes compared to soluble ligand. We therefore examined the effect of tethered EGF on osteogenic colony formation from human bone marrow aspirates in the context of three different adhesion environments using a total of 39 donors. We found that tethered EGF, but not soluble EGF, increased the numbers of colonies formed regardless of adhesion background, and that tethered EGF did not impair early stages of osteogenic differentiation. National Institute of General Medical Sciences (U.S.) (Grant NIH RO1 AR42997) National Institute of General Medical Sciences (U.S.) (Grant NIH RO1 AG024980) National Institute of General Medical Sciences (U.S.) (Grant NIH RO1 GM59870) National Institute of General Medical Sciences (U.S.) (Grant NIH DE019523) |
| Databáze: | OpenAIRE |
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