Time-dependent effects of Pochonia chlamydosporia endophytism on gene expression profiles of colonized tomato roots
| Autor: | Laura Cristina Rosso, Aurelio Ciancio, Isabella Pentimone, Franco Nigro, Mariantonietta Colagiero, Massimo Ferrara |
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| Rok vydání: | 2019 |
| Předmět: |
Time Factors
Hypocreales Fungus Applied Microbiology and Biotechnology Plant Roots Transcriptome 03 medical and health sciences Solanum lycopersicum Gene Expression Regulation Plant Gene Expression Regulation Fungal Gene expression Plant defense against herbivory Endophytes Colonization Plant defense Pochonia chlamydosporia Root endophytism Signaling Tomato Transcription factor Gene 030304 developmental biology Genetics 0303 health sciences biology Host Microbial Interactions 030306 microbiology General Medicine biology.organism_classification Biotechnology |
| Zdroj: | Applied microbiology and biotechnology (2019). doi:10.1007/s00253-019-10058-z info:cnr-pdr/source/autori:Pentimone I., Colagiero M., Ferrara M., Nigro F., Rosso LC., Ciancio A./titolo:Time-dependent effects of Pochonia chlamydosporia endophytism on gene expression profiles of colonized tomato roots./doi:10.1007%2Fs00253-019-10058-z/rivista:Applied microbiology and biotechnology/anno:2019/pagina_da:/pagina_a:/intervallo_pagine:/volume |
| ISSN: | 1432-0614 |
| Popis: | A transcriptome analysis was produced from tomato roots inoculated with the hyphomycete Pochonia chlamydosporia at three different times. Gene expression data were also yielded from fungus grown in vitro or endophytic. A next-generation sequencing (NGS) and network analysis approach were applied. We identified 3.676 differentially expressed tomato genes (DEG), highlighting a core of 93 transcripts commonly down- or upregulated at every time point, shedding light on endophytism process. Functional categories related to plant information-processing system, which recognizes, percepts, and transmits signals, were associated with gene upregulated early in time, with higher representations in processes such as plant defense regulation later in time. Network analysis of a DEG subset showed dominance of MAP kinase hubs in the uninoculated control samples, replaced by an increased centrality of WRKY transcription factor and ETR-ethylene response factor genes in the colonized roots. Fungus genes expressed during progression of plant colonization, therefore related to the host colonization process or endophytism persistence, were also identified. Data provided a high-resolution insight on tomato transcriptome changes as induced by endophytism, highlighting a specific modulation of stress-responsive transcripts, related to a selective activation of defense pathways, likely required by the fungus to establish a persistent endophytic lifestyle. |
| Databáze: | OpenAIRE |
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