Identification of 19-nor-corticosterone in rat urine
| Autor: | M.M Holbrook, F.B Field, S.L Dale, J.C Melby, F Aladar Bencsath |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Urinary system
Clinical Biochemistry Urine Biology Rats Inbred WKY Biochemistry High-performance liquid chromatography Gas Chromatography-Mass Spectrometry chemistry.chemical_compound Endocrinology Corticosterone Animals Desoxycorticosterone Molecular Biology Chromatography High Pressure Liquid Pharmacology Chromatography Organic Chemistry Acetylation Metabolism Rats chemistry Female Free form Derivative (chemistry) |
| Zdroj: | Steroids. 45:91-98 |
| ISSN: | 0039-128X |
| Popis: | 19-Nor-corticosterone (19-nor-B) was isolated from a pool of urine collected from Wistar-Kyoto (WKY) rats. Identity was established by comparison of chromatographic mobilities with standard 19-nor-B both as the free compound and diacetate derivative. Comparison of the gas chromatographic-mass spectra of the urinary product and standard 19-nor-B as the diacetate following isolation by high pressure liquid chromatography (HPLC) confirmed the identity of the urinary product to be 19-nor-B. This demonstration that 19-nor-B is a naturally occuring product in addition to 19-nor-deoxycorticosterone supports the concept of the existence of a biosynthetic pathway for the formation of the 19-nor-corticosteroids. |
| Databáze: | OpenAIRE |
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