Arabidopsis Basic Helix-Loop-Helix 34 (bHLH34) Is Involved in Glucose Signaling through Binding to a GAGA Cis-Element
| Autor: | Hyun-Woo Ju, Cheol Soo Kim, Kyeong-Hwan Lee, Ji-Hee Min, Dayoung Yoon, Sungbeom Lee |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Regulator macromolecular substances Plant Science lcsh:Plant culture 01 natural sciences activator 03 medical and health sciences chemistry.chemical_compound Transcription (biology) Arabidopsis lcsh:SB1-1110 Transcription factor Gene Abscisic acid transcription factor Original Research bHLH34 biology Basic helix-loop-helix Chemistry fungi AtPGR food and beverages Promoter biology.organism_classification Cell biology carbohydrates (lipids) 030104 developmental biology glucose-responsive element lipids (amino acids peptides and proteins) 010606 plant biology & botany |
| Zdroj: | Frontiers in Plant Science, Vol 8 (2017) Frontiers in Plant Science |
| ISSN: | 1664-462X |
| DOI: | 10.3389/fpls.2017.02100 |
| Popis: | The modulation of glucose (Glc) homeostasis and signaling is crucial for plant growth and development. Nevertheless, the molecular signaling mechanism by which a plant senses a cellular Glc level and coordinates the expression of Glc-responsive genes is still incompletely understood. Previous studies have shown that Arabidopsis thaliana plasma membrane Glc-responsive regulator (AtPGR) is a component of the Glc-responsive pathway. Here, we demonstrated that a transcription factor bHLH34 binds to 5′-GAGA-3′ element of the promoter region of AtPGR in vitro, and activates beta-glucuronidase (GUS) activity upon Glc treatment in AtPGR promoter-GUS transgenic plants. Gain- and loss-of-function analyses suggested that the bHLH34 involved in the responses to not only Glc, but also abscisic acid (ABA) and salinity. These results suggest that bHLH34 functions as a transcription factor in the Glc-mediated stress responsive pathway as well as an activator of AtPGR transcription. Furthermore, genetic experiments revealed that in Glc response, the functions of bHLH34 are different from that of a bHLH104, a homologue of bHLH34. Collectively, our findings indicate that bHLH34 is a positive regulator of Glc, and may affect ABA or salinity response, whereas bHLH104 is a negative regulator and epistatic to bHLH34 in the Glc response. |
| Databáze: | OpenAIRE |
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