Expressions of Matrix Metalloproteinases-9 and Tissue Inhibitor of Metalloproteinase-1 in Pituitary Adenomas and Their Relationships with Prognosis
| Autor: | Yi Xiang, Lianfeng Guo, Jianhui Mao, Jianhui Wei, Zhanyi Xu, Lei Qiu, Zhaosheng Sun, Wangmiao Zhao, Hong Guo |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Adenoma
Adult Male 0301 basic medicine Cancer Research Matrix (biology) Matrix metalloproteinase 03 medical and health sciences 0302 clinical medicine Pituitary adenoma Biomarkers Tumor medicine Humans Pituitary Neoplasms Radiology Nuclear Medicine and imaging Pharmacology Tissue Inhibitor of Metalloproteinase-1 Chemistry Matrix metalloproteinase 9 General Medicine Middle Aged Tissue inhibitor of metalloproteinase Prognosis medicine.disease Immunohistochemistry 030104 developmental biology Matrix Metalloproteinase 9 Oncology 030220 oncology & carcinogenesis Cancer research Female |
| Zdroj: | Cancer Biotherapy and Radiopharmaceuticals. 34:1-6 |
| ISSN: | 1557-8852 1084-9785 |
| Popis: | To investigate the expression levels of matrix metalloproteinases-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in pituitary adenomas (PAs), and to analyze the relationship of the expressions of the two with the prognosis of patients.A total of 108 patients with PAs diagnosed in our hospital from May 2010 to May 2012 were selected and divided into the invasive PA (IPA) group (n = 58) and the non-IPA group (n = 50) according to the invasiveness of PAs. Hematoxylin and eosin (HE) staining was used to observe the pathological state of patients. The expression levels of MMP-9 and TIMP-1 were measured by immunohistochemistry and western blotting at protein level and reverse transcription-polymerase chain reaction at gene level, respectively. The expression levels of MMP-9 and TIMP-1 in serum of patients before operation were tested using enzyme-linked immunosorbent assay, and patients with PAs after operation were followed up.The positive expression rate of MMP-9 in IPAs was significantly higher than that in non-IPAs, whereas that of TIMP-1 was relatively high in non-IPAs, and the differences were statistically significant (p 0.05). At both protein and gene levels, MMP-9 was highly expressed in IPAs, whereas TIMP-1 was highly expressed in non-IPAs, and the differences were statistically significant (p 0.05 in all comparisons). Before operation, the expression level of MMP-9 in serum of patients with IPAs was relatively high, whereas that of TIMP-1 in serum of patients with non-IPAs was relatively high, and the differences were statistically significant (p 0.05 in all comparisons).The postoperative survival rate of patients with highly expressed MMP-9 was relatively low, whereas that of patients with highly expressed TIMP-1 was relatively high. The abnormal expressions of MMP-9 and TIMP-1 play important roles in the invasion process of PAs. The prognoses of patients with low expression MMP-9 and high expression TIMP-1 are more positive. |
| Databáze: | OpenAIRE |
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