Increased CD34+/KDR+ cells are not associated with carotid artery intima-media thickness progression in chronic HIV-positive subjects
| Autor: | Maxwell Pistilli, Jeffrey N. Martin, Priscilla Y. Hsue, Emmanouil Papasavvas, Griffin Reynolds, Steven G. Deeks, Luis J. Montaner, Aidan Hancock |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Tunica media medicine.medical_specialty Endothelium CD34 Antigens CD34 HIV Infections Carotid Intima-Media Thickness Peripheral blood mononuclear cell Gastroenterology Article Internal medicine medicine Humans Pharmacology (medical) Progenitor cell Pharmacology business.industry Stem Cells Endothelial Cells Middle Aged Atherosclerosis Flow Cytometry Tunica intima Carotid Arteries Infectious Diseases medicine.anatomical_structure Intima-media thickness Chronic Disease Cohort Disease Progression cardiovascular system Cardiology Female Endothelium Vascular Tunica Intima Tunica Media business |
| Zdroj: | Antiviral Therapy. 17:557-563 |
| ISSN: | 2040-2058 1359-6535 |
| DOI: | 10.3851/imp2013 |
| Popis: | Background Endothelial progenitor cells (EPCs) are involved in the endothelium repair. Low circulating EPC levels are predictive of cardiovascular events in HIV-negative subjects. The impact of HIV infection on EPCs, and the role of EPCs in HIV-associated cardiovascular disease, is not known. We hypothesized that circulating EPCs would be inversely associated with carotid artery intima-media thickness (c-IMT) changes in HIV-infected subjects. Methods EPCs (CD34+/KDR+, CD133+/KDR+ and CD34+/ CD133+/KDR+) were defined retrospectively by flow cytometry in cryopreserved peripheral blood mononuclear cells collected longitudinally from 66 chronic HIV-infected subjects and cross-sectionally from 50 at-risk HIV- negative subjects. The HIV-infected subjects participated in the Study of the Consequences of the Protease Inhibitor Era (SCOPE) cohort, were receiving antiretroviral therapy (59/66) and had two sequential measurements of c-IMT 1 year apart. Two distinct groups of HIV-infected subjects were identified a priori: rapid c-IMT progressors (subjects with rapid c-IMT progression, n=13, Δc-IMT>0.2 mm) and slow c-IMT progressors (subjects with slow or no c-IMT progression, n=53, Δc-IMTResults Although cryopreservation reduced sensitivity of detection, EPC frequency in HIV-infected subjects was still significantly higher compared to at-risk HIV-negative subjects (CD34+/KDR+; P=0.01) and correlated positively with CD4+ T-cell count (CD34+/KDR+, r=0.27; P=0.03). No association was found between the change of EPC frequencies over time (ΔEPC) and Δc-IMT or between EPC frequencies and c-IMT or Δc-IMT. Conclusions The lack of an association between EPCs and c-IMT in our cohort does not support HIV-associated reductions in EPC frequency as a cause of accelerated atherosclerosis. |
| Databáze: | OpenAIRE |
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