Interactions of dietary fat and 2,5-anhydro-d -mannitol on energy metabolism in isolated rat hepatocytes
| Autor: | Mary Osbakken, Hong Ji, Grazyna Graczyk-Milbrandt, Mark I. Friedman |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Male
medicine.medical_specialty Magnetic Resonance Spectroscopy Physiology Palmitates Energy metabolism Cell Separation Fructose Biology Rats Sprague-Dawley chemistry.chemical_compound Adenosine Triphosphate Oxygen Consumption Physiology (medical) Internal medicine Dietary Carbohydrates medicine Animals Mannitol Dietary fat Phosphorus Carbohydrate Dietary Fats In vitro Rats Endocrinology medicine.anatomical_structure chemistry Biochemistry D-mannitol Hepatocyte Hepatocytes Energy Metabolism Oxidation-Reduction Adenosine triphosphate |
| Zdroj: | American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 282:R715-R720 |
| ISSN: | 1522-1490 0363-6119 |
| DOI: | 10.1152/ajpregu.00159.2001 |
| Popis: | The fructose analog 2,5-anhydro-d-mannitol (2,5-AM) stimulates feeding in rats by reducing ATP content in the liver. These behavioral and metabolic effects occur with rats fed a high-carbohydrate/low-fat (HC/LF) diet, but they are prevented or attenuated when the animals eat high-fat/low-carbohydrate (HF/LC) food. To examine the metabolic bases for this effect of diet, we assessed the actions of 2,5-AM on ATP content, oxygen consumption, and substrate oxidation in isolated hepatocytes from rats fed one of the two diets. Compared with cells from rats fed the HC/LF diet (“HC/LF” cells), cells from rats fed the HF/LC diet (“HF/LC” cells) had similar ATP contents but lower oxygen consumption, decreased fructose, and increased palmitate oxidation. 2,5-AM did not decrease ATP content or oxygen consumption in HF/LC cells as much as it did in HC/LF hepatocytes, and it only affected fructose and palmitate oxidation in HC/LF cells.31P-NMR spectroscopy indicated that differences in phosphate trapping accounted for differences in depletion of ATP by 2,5-AM. These results suggest that intake of the HF/LC diet prevents the eating response and attenuates the decline in liver ATP by shifting hepatocyte metabolism to favor fat over carbohydrate as an energy-yielding substrate. |
| Databáze: | OpenAIRE |
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