Effect of Zincum metallicum and LPS treatments on femoral bone density in pregnant mice
| Autor: | Carla Holandino, Silvio Leite Monteiro da Silva, Maria Martha Bernardi, Vanessa Gallego Arias Pecorari, Leoni Villano Bonamin, Claudio Costa, Emiko Saito Arita, Maristela Dutra Correa |
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| Rok vydání: | 2021 |
| Předmět: |
Pregnancy
Serial dilution biology Lipopolysaccharide business.industry medicine.disease biology.organism_classification BALB/c Lead preparation Andrology chemistry.chemical_compound medicine.anatomical_structure Complementary and alternative medicine chemistry Lactation Immunology medicine Gestation Lactose business |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1982-6206 |
| Popis: | Background: The simulated bacterial infection is a classical experimental model, by the injection of purified gram negative bacteria wall lipopolysaccharide (LPS) which produces effects similar to real infection, but without presence of the microorganism. The LPS is a stress factor as during the pregnancy and delivery, and pilot experiments have shown that ultra-high dilutions (UHD) may have a beneficial role in reducing the LPS stress. Zincum metallicum (Zm) has a potential to influence several physiological aspects of a living organism. It was chosen by a team of researchers from GIRI, to explore the effect of Zm on various experimental model (Zincum project). The optical density is a radiographic method for the measurement of the amount of hard bone tissue, and is an accessible, simple and non-invasive method. It is hypothesized that UHD Zm may influence the Zinc metabolism, therefore, bone tissues were collected to evaluate the zinc concentration. Methods: This project was previously approved by the animal ethical committee vide protocol#156/13 by CEUA-UNIP. 28 parental female generation (Pf) BALB/c mice from Universidade de Sao Paulo (Brazil) were divided in four blinded groups (n=7 for each). The mice were treated by the vehicle Lactosis 6cH (potentized lactose) as control; Zm 6cH, 30cH and 200cH separately through drinking water ad libitum during 31 days: 21 from the mate to the delivery and more 10 days of lactation. The potencies were chosen to cover a classical range of common use. The intraperitoneal LPS injection was made in Pf between 9th and 10th day of gestation day (n=5 to 6 per group). Some Pf gave birth and all its F1 sons were separated by sex, grown up to two months till adulthood, and exposed to LPS a day prior to right femur collection for analysis. All the 71 collected samples were conserved cold and were labelled blind. They were exposed using a portable X-ray machine (Nomad® Aribex, USA). Images were analysed by an indirect digital scanner (Soredex Digora® Optime) with photostimulable phosphor plates (PSP) (Figure-Ia and |
| Databáze: | OpenAIRE |
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