Co-Adjuvant Therapy Efficacy of Catechin and Procyanidin B2 with Docetaxel on Hormone-Related Cancers In Vitro

Autor: José-Luis Santiago, María-Carmen Martínez, Mª Elena Pérez-Muñuzuri, Mª Jesús Núñez-Iglesias, S. Boso, Pilar Gago, Silvia Novío, Carlota García, Manuel Freire-Garabal
Rok vydání: 2021
Předmět:
0301 basic medicine
Male
Docetaxel
Pharmacology
Catechin
chemistry.chemical_compound
plant co-adjuvant therapy
0302 clinical medicine
Prostate
natural substances
Biology (General)
Procyanidin B2
Spectroscopy
Sensitization
General Medicine
Computer Science Applications
Chemistry
medicine.anatomical_structure
030220 oncology & carcinogenesis
procyanidin
MCF-7 Cells
Drug Therapy
Combination
Female
medicine.drug
QH301-705.5
Antineoplastic Agents
Breast Neoplasms
In Vitro Techniques
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
Breast cancer
DU145
medicine
Adjuvant therapy
Biflavonoids
Humans
antitumor activity
Proanthocyanidins
Physical and Theoretical Chemistry
Adverse effect
Molecular Biology
QD1-999
docetaxel-sensitization
business.industry
hormone-related cancer
Organic Chemistry
Prostatic Neoplasms
medicine.disease
030104 developmental biology
chemistry
Drug Screening Assays
Antitumor
business
Zdroj: International Journal of Molecular Sciences
International Journal of Molecular Sciences, Vol 22, Iss 7178, p 7178 (2021)
Volume 22
Issue 13
ISSN: 1422-0067
Popis: Prostate (PC) and breast cancer (BC) are heterogeneous hormonal cancers. Treatment resistance and adverse effects are the main limitations of conventional chemotherapy treatment. The use of sensitizing agents could improve the effectiveness of chemotherapeutic drugs as well as obviate these limitations. This study analyzes the effect of single catechin (CAT), procyanidin B2 (ProB2) treatment as well as the co-adjuvant treatment of each of these compounds with docetaxel (DOCE). We used PC- and BC-derived cell lines (PC3, DU-145, T47D, MCF-7 and MDA-MB-231). The short and long-term pro-apoptotic, anti-proliferative and anti-migratory effects were analyzed. RT-qPCR was used to discover molecular bases of the therapeutic efficacy of these compounds. ProB2 treatment induced a two- to five-fold increase in anti-proliferative and pro-apoptotic effects compared to single DOCE treatment, and also had a more sensitizing effect than DOCE on DU145 cells. Regarding BC cells, ProB2- and CAT-mediated sensitization to DOCE anti-proliferative and pro-apoptotic effects was cell-independent and cell-dependent, respectively. Combined treatment led to high-efficacy effects on MCF-7 cells, which were associated to the up-regulation of CDKN1A, BAX, caspase 9 and E-cadherin mRNA under combined treatment compared to single DOCE treatment. CAT and ProB2 can enhance the efficacy of DOCE therapy on PC and BC cells by the sensitizing mechanism.
Databáze: OpenAIRE
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