Micronuclei and Nuclear Buds Induced by Cyclophosphamide in Crocodylus moreletii as Useful Biomarkers in Aquatic Environments

Autor: Belinda C. Gómez-Meda, Guillermo Moisés Zúñiga-González, Martha Patricia Gallegos-Arreola, Ana Lourdes Zamora-Perez, Blanca Miriam Torres-Mendoza, Juan Ernesto Gutiérrez-Sevilla, Jaime Luna-Aguirre, Ramón Guillermo Ortiz-García, Olivia Torres-Bugarín
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Animals : an Open Access Journal from MDPI
Animals, Vol 11, Iss 3178, p 3178 (2021)
Animals
Volume 11
Issue 11
ISSN: 2076-2615
Popis: Micronuclei (MN) are used to assess genotoxic exposure, whereas nuclear buds (NBs) have been linked to genotoxic events. Crocodylus moreletii was studied to identify MN and NBs. Three groups were formed: Group 1 (water) and groups 2 and 3 (7 or 10 mg/kg of cyclophosphamide). A drop of blood was obtained daily from the claw tip at 0 to 120 h. Spontaneous micronucleated erythrocytes (MNEs) and erythrocytes with nuclear buds (NBEs) were counted. The frequencies of micronucleated young erythrocytes (MNYEs) and NB young erythrocytes (NBYEs) were evaluated, including the ratio of young erythrocytes (YE)/1000 total erythrocytes. No significant differences were observed in the YE proportion on sampling days
group 1 did not show differences for any parameter, whereas group 2 showed significant differences in MNEs and NBEs, and group 3 showed differences in NBEs and NBYEs. Some mitotic activity in circulation was observed in YEs. In conclusion, NBEs could be a more sensitive biomarker to genotoxic damage than MNEs. The identification of these biomarkers leads us to propose Crocodylus moreletii as a possible environment bioindicator because these parameters could be useful to analyze the in vivo health status of these reptiles and for biomonitoring genotoxic pollutants in their habitats.
Databáze: OpenAIRE
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