Use of nano-liquid chromatography for the analysis of glycyrrhizin and glycyrrhetic acid in licorice roots and candies
| Autor: | Maria Augusta Raggi, Salvatore Fanali, Zeineb Aturki, Giovanni D'Orazio, M.G. Quaglia, Cesare Sabbioni, Anna Rocco |
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| Přispěvatelé: | S. Fanali, Z. Aturki, G. D’Orazio, M.A. Raggi, M.G. Quaglia, C. Sabbioni, A. Rocco |
| Rok vydání: | 2005 |
| Předmět: |
glycyrrhizin
Saponin Filtration and Separation Uronic acid High-performance liquid chromatography Plant Roots Sensitivity and Specificity Analytical Chemistry miniaturization chemistry.chemical_compound licorice glycyrrhetic acid Phase (matter) Glycyrrhiza Nanotechnology Glycyrrhizin Enoxolone acids Chromatography High Pressure Liquid chemistry.chemical_classification Chromatography food Glycoside Reproducibility of Results Reversed-phase chromatography Glycyrrhizic Acid chemistry Glycyrrhetinic Acid Spectrophotometry Ultraviolet nano-liquid chromatography |
| Zdroj: | Journal of separation science 28 (2005): 982–986. doi:10.1002/jssc.200400096 info:cnr-pdr/source/autori:Fanali, S; Aturki, Z; D'Orazio, G; Raggi, MA; Quaglia, MG; Sabbioni, C; Rocco, A/titolo:Use of nano-liquid chromatography for the analysis of glycyrrhizin and glycyrrhetic acid in licorice roots and candies/doi:10.1002%2Fjssc.200400096/rivista:Journal of separation science (Print)/anno:2005/pagina_da:982/pagina_a:986/intervallo_pagine:982–986/volume:28 28 (2005): 982. info:cnr-pdr/source/autori:S. Fanali, Z. Aturki, G. DOrazio, M. A. Raggi, M. G. Quaglia, C. Sabbioni, A. Rocco/titolo:Use of nano-liquid chromatography for the analysis of glycyrrhizin and glycyrrhetic acid in licorice roots and candies/doi:/rivista:/anno:2005/pagina_da:982/pagina_a:/intervallo_pagine:982/volume:28 |
| ISSN: | 1615-9306 |
| DOI: | 10.1002/jssc.200400096 |
| Popis: | Glycyrrhizin (G) and glycyrrhetic acid (GA) were separated by using nano-liquid chromatography(nano-LC) in a fused silica capillary packed with RP18 stationary phase (75 micron ID, effective length 33 cm, packed 23 cm) eluting at 300 nL/min in a gradient mode. The mobile phase was a mixture of water-MeOH-MeCN-acetic acid (29:35:35:1, v/v/v/v) that was delivered for one minute and after this was modified by reducing the water content (14 :42.5 :42.5 :1, v/v/v/v). The intra-day and inter-day relative standard deviations (of retention time and peak area) were satisfactory (lower than 2.9 and 4%, respectively). The linearity of the nano-LC method was assessed in the range 0.625.00 microg/mL and 80200 microg/mL for GA and G, with R2 = 0.996 and 0.995, respectively. The licorice was extracted with a mixture of ethanol-water, diluted with the mobile phase, and injected for the analysis. |
| Databáze: | OpenAIRE |
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