Inhibition of let-7c Regulates Cardiac Regeneration after Cryoinjury in Adult Zebrafish

Autor: Jere Paavola, Hong Wang, Heikki Ruskoaho, Katariina Immonen, Mika Laine, Sanni Perttunen, Karri Kalervo, Riikka Kosonen, Ilkka Tikkanen, Suneeta Narumanchi, Päivi Lakkisto
Přispěvatelé: Medicum, HUS Heart and Lung Center, Kardiologian yksikkö, University of Helsinki, Regenerative pharmacology group, Drug Research Program, Division of Pharmacology and Pharmacotherapy, Department of Medicine, HUS Abdominal Center, University Management, Nefrologian yksikkö, Neurologian yksikkö, Department of Neurosciences, HUS Neurocenter, University of Helsinki, Regenerative pharmacology group, University of Helsinki, Department of Medicine, University of Helsinki, Department of Clinical Chemistry and Hematology
Rok vydání: 2019
Předmět:
Cardiac function curve
ENDOCARDIUM
lcsh:Diseases of the circulatory (Cardiovascular) system
viruses
animal diseases
medicine.medical_treatment
education
MICRORNA THERAPEUTICS
CAPACITY
Andrology
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
parasitic diseases
microRNA
Fibrinolysis
medicine
Pharmacology (medical)
Antagomir
fibrin
General Pharmacology
Toxicology and Pharmaceutics
Zebrafish
030304 developmental biology
let-7c
SEPT7B
proliferating cardiomyocytes
0303 health sciences
biology
Regeneration (biology)
Embryogenesis
cardiac regeneration
virus diseases
biology.organism_classification
FAMILY
Proliferating cell nuclear antigen
DIFFERENTIATION
EPICARDIUM
chemistry
CARDIOVASCULAR-DISEASE
lcsh:RC666-701
3121 General medicine
internal medicine and other clinical medicine
CELLS
miRNAs
biology.protein
3111 Biomedicine
epicardial cells
030217 neurology & neurosurgery
HEART REGENERATION
Zdroj: Journal of Cardiovascular Development and Disease
Volume 6
Issue 2
Journal of Cardiovascular Development and Disease, Vol 6, Iss 2, p 16 (2019)
ISSN: 2308-3425
DOI: 10.3390/jcdd6020016
Popis: The let-7c family of micro-RNAs (miRNAs) is expressed during embryonic development and plays an important role in cell differentiation. We have investigated the role of let-7c in heart regeneration after injury in adult zebrafish. let-7c antagomir or scramble injections were given at one day after cryoinjury (1 dpi). Tissue samples were collected at 7 dpi, 14 dpi and 28 dpi and cardiac function was assessed before cryoinjury, 1 dpi, 7 dpi, 14 dpi and 28 dpi. Inhibition of let-7c increased the rate of fibrinolysis, increased the number of proliferating cell nuclear antigen (PCNA) positive cardiomyocytes at 7 dpi and increased the expression of the epicardial marker raldh2 at 7 dpi. Additionally, cardiac function measured with echocardiography recovered slightly more rapidly after inhibition of let-7c. These results reveal a beneficial role of let-7c inhibition in adult zebrafish heart regeneration.
Databáze: OpenAIRE
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