The genetic heterozygosity and fitness of tetraploid embryos and embryonic stem cells are crucial parameters influencing survival of mice derived from embryonic stem cells by tetraploid embryo aggregation
| Autor: | Jun Yong, Xiangyun Li, Yuansong Yu, Qing Jia, Keqian Di, Wei Wei |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Genetic Markers
Heterozygote Embryology Embryonic Development Mice Inbred Strains Hybrid Cells Biology Polymerase Chain Reaction Germline Embryo Culture Techniques Polyploidy Loss of heterozygosity Mice Endocrinology Species Specificity Animals Genetics Tetraploid complementation assay Stem Cells food and beverages Obstetrics and Gynecology Embryo DNA Cell Biology Embryo Mammalian Phenotype Embryonic stem cell Genetically modified organism Reproductive Medicine Embryo Loss Female Stem cell Microsatellite Repeats |
| Zdroj: | Reproduction. 130:53-59 |
| ISSN: | 1741-7899 1470-1626 |
| DOI: | 10.1530/rep.1.00667 |
| Popis: | The aim of this paper was to determine whether the genetic background of tetraploid embryos contributed to the survival of mice derived from embryonic stem (ES) cells by tetraploid embryo complementation. Twenty-five newborns were produced by aggregation of hybrid ES cells and tetraploid embryos with different genetic backgrounds. These newborns were entirely derived from ES cells judged by microsatellite DNA (A specific sequence of DNA bases or nucleotides that contains mono, di, tri or tetra repeats) and coat colour phenotype and germline transmission. Fifteen survived to adulthood while seven died of respiratory failure. All newborns were derived from outbred or hybrid tetraploid aggregates and no newborns were from the inbreds. Our results demonstrate that the genetic heterozygosity, fitness of tetraploid embryos and fitness of ES cells are crucial parameters influencing survival of mice derived from ES cells by tetraploid embryo aggregation. In addition, this method represents a simple and efficient procedure for immediate generation of targeted mouse mutants from genetically modified ES cell clones, in contrast to the standard protocol, which involves the production of chimeras and several breeding steps. |
| Databáze: | OpenAIRE |
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