Pex8p: an intraperoxisomal organizer of the peroxisomal import machinery
| Autor: | Wolf H. Kunau, Birgit Agne, Helmut E. Meyer, Nadja M Meindl, Karsten Niederhoff, Albert Sickmann, Henrik Einwächter, Wolfgang Girzalsky, Peter Rehling |
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| Rok vydání: | 2003 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Peroxisome-Targeting Signal 1 Receptor Recombinant Fusion Proteins Ubiquitin-Protein Ligases Peroxisomal Biogenesis Factor 2 Receptors Cytoplasmic and Nuclear Peroxin Saccharomyces cerevisiae Biology Models Biological Fungal Proteins Peroxins Cytosol Ring finger medicine Peroxisomes Molecular Biology Peroxisomal matrix Cell Membrane Membrane Proteins Membrane Transport Proteins Cell Biology Peroxisome Cell biology Repressor Proteins medicine.anatomical_structure Biochemistry PEX1 Electrophoresis Polyacrylamide Gel Carrier Proteins PEX6 Plasmids Protein Binding |
| Zdroj: | Molecular cell. 11(3) |
| ISSN: | 1097-2765 |
| Popis: | Peroxisomes transport folded and oligomeric proteins across their membrane. Two cytosolic import receptors, Pex5p and Pex7p, along with approximately 12 membrane-bound peroxins participate in this process. While interactions among individual peroxins have been described, their organization into functional units has remained elusive. We have purified and defined two core complexes of the peroxisomal import machinery: the docking complex comprising Pex14p and Pex17p, with the loosely associated Pex13p, and the RING finger complex containing Pex2p, Pex10p, and Pex12p. Association of both complexes into a larger import complex requires Pex8p, an intraperoxisomal protein. We conclude that Pex8p organizes the formation of the larger import complex from the trans side of the peroxisomal membrane and thus might enable functional communication between both sides of the membrane. |
| Databáze: | OpenAIRE |
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