Detection of rabbit haemorrhagic disease virus isolates and sequence comparison of the N-terminus of the capsid protein gene by the polymerase chain reaction
| Autor: | C. Trepo, M. Prave, G Le Gall, I. Baginski, C. Guittré, Lucyna Cova |
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| Rok vydání: | 1995 |
| Předmět: |
Hemorrhagic Disease Virus
Rabbit Sequence analysis viruses Molecular Sequence Data Polymerase Chain Reaction Virus law.invention Rabbit haemorrhagic disease Capsid law Sequence Homology Nucleic Acid Complementary DNA Animals Amino Acid Sequence Peptide sequence Polymerase chain reaction Base Sequence Sequence Homology Amino Acid General Veterinary biology Sequence Analysis DNA biology.organism_classification Virology Molecular biology Reverse transcriptase Rabbits |
| Zdroj: | Research in Veterinary Science. 58:128-132 |
| ISSN: | 0034-5288 |
| DOI: | 10.1016/0034-5288(95)90065-9 |
| Popis: | At present there is no sensitive method for the detection of rabbit haemorrhagic disease virus (RHDV), a calicivirus causing high mortality in rabbit populations. For this purpose a reverse transcriptase polymerase chain reaction (RT-PCR) was established in the N-terminal portion of the RHDV capsid region. The RT-PCR was 10(4)-fold more sensitive than ELISA testing for the detection of the virus and was able to detect as few as 12 copies of template cDNA. By using the RT-PCR test and sequencing, 96.6 to 98.7 per cent homology was demonstrated in the N-terminal portion of the capsid protein of three isolates from geographically and temporally separate outbreaks of viral haemorrhagic disease, indicating that this portion of the RHDV capsid protein is highly conserved. |
| Databáze: | OpenAIRE |
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