Encapsidation and expression of the herpes thymidine kinase gene in polyoma virus
Autor: | N B Dinces, G Milman |
---|---|
Rok vydání: | 1982 |
Předmět: |
Thymidine kinase activity
Genes Viral viruses Immunology DNA Recombinant Biology Transfection medicine.disease_cause Origin of replication Thymidine Kinase Microbiology law.invention Mice Viral Proteins Viral entry law Virology Operon medicine Viral structural protein Animals Simplexvirus Antigens Viral Tumor Antigens Viral Structural gene Molecular biology Herpes simplex virus Gene Expression Regulation Thymidine kinase Insect Science DNA Viral Recombinant DNA Polyomavirus Research Article |
Zdroj: | Journal of Virology. 42:691-699 |
ISSN: | 1098-5514 0022-538X |
Popis: | A recombinant DNA of 5,150 base pairs was prepared containing the intact early region of polyoma virus, including the viral origin of replication and the structural sequences of the herpes simplex virus type 1 thymidine kinase gene. Although no thymidine kinase activity was detected when herpes structural sequences alone were transfected into cells, activity was produced when the structural gene followed the polyoma early region. The recombinant DNA was encapsidated into polyoma virions when cotransfected into mouse 3T6 cells with helper DNA from an early polyoma virus mutant. Herpes thymidine kinase activity was detected by a rapid in situ autoradiographic assay in which [125]iododeoxycytidine was utilized as a substrate for the viral but not the cellular enzyme. |
Databáze: | OpenAIRE |
Externí odkaz: |