Anticancer potentiality of lignan rich fraction of six Flaxseed cultivars
Autor: | Abeer A. Elkhoely, Abeer A. El Sayed, Yasmin M. Attia, Mohamed S. Elsesy, Nebal El Tanbouly, Samia A. Shouman, Mouchira A. Choucry, Essam Abdel Sattar, Sabah H. El Gayed, Amira S. El Senousy, Shahira M. Ezzat |
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Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Angiogenesis medicine.medical_treatment lcsh:Medicine Antineoplastic Agents Apoptosis Pharmacology Article Lignans Hydrolysate Ehrlich ascites carcinoma Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Flax Biomarkers Tumor medicine Animals Humans lcsh:Science IC50 Lignan Multidisciplinary Plant Extracts Growth factor lcsh:R Mammary Neoplasms Experimental HCT116 Cells Vascular endothelial growth factor 030104 developmental biology chemistry 030220 oncology & carcinogenesis MCF-7 Cells Female lcsh:Q HeLa Cells |
Zdroj: | Scientific Reports, Vol 8, Iss 1, Pp 1-16 (2018) Scientific Reports |
ISSN: | 2045-2322 |
DOI: | 10.1038/s41598-017-18944-0 |
Popis: | The objective of our study is to highlight the therapeutic effect and mechanism of action by which purified Flaxseed hydrolysate (PFH) which is a lignan rich fraction exerts its anticancer activity on a human breast cancer cell line (T47D) and in mice bearing tumor. HPLC analysis of PFH of six flaxseed cultivars had shown that PFH of the cultivar Giza 9 (PFH-G9) contains the highest concentration of SDG (81.64 mg/g). The in vitro cytotoxic potentiality of PFH’s of six flaxseed cultivars was screened against a panel of human cancer cell lines. PFH -G9 showed the most significant cytotoxic activity against ER-receptor positive breast cell lines MCF7 and T47D with IC50 13.8 and 15.8 µg/ml, respectively. Moreover, PFH-G9 reduced the expression of the metastasis marker, 1-α, metalloproteinases and vascular endothelial growth factor (VEGF), one of the most potent stimulators of angiogenesis, while it increased the caspase-3 dependent apoptosis. Our study also showed that dietary intake of 10% of Giza 9 Flaxseeds (FS), fixed oil (FSO) or Flax meal (FSM) twice daily for 3 weeks in mice-bearing solid Ehrlich ascites carcinoma (EAC) resulted in reducing the tumor volume, the expression of estrogen, insulin growth factor, progesterone, VEGF and MMP-2, but enhanced expression of caspase-3. |
Databáze: | OpenAIRE |
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