Relationships between metabolic profiles and gene expression in liver and leukocytes of dairy cows in early lactation
Autor: | Haruko Takeda, Cinzia Marchitelli, Laura Buggiotti, Zhangrui Cheng, D Claire Wathes, Miel Hostens, Tine Rousing, Mazdak Salavati, Federica Signorelli, Mark Crowe, Klaus Lønne Ingvartsen, Leslie Foldager, Lijing Tang |
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Rok vydání: | 2020 |
Předmět: |
medicine.medical_specialty
metabolic clustering Population PDK4 Gene Expression Biology Fatty Acids Nonesterified liver Transcriptome 03 medical and health sciences Insulin resistance Pregnancy negative energy balance Lactation Internal medicine Gene expression Genetics medicine Leukocytes Glucose homeostasis Animals education 030304 developmental biology 2. Zero hunger 0303 health sciences education.field_of_study 3-Hydroxybutyric Acid 0402 animal and dairy science food and beverages RNA sequencing Lipid metabolism 04 agricultural and veterinary sciences medicine.disease 040201 dairy & animal science Diet medicine.anatomical_structure Endocrinology Milk Liver Metabolome Animal Science and Zoology Cattle Female Energy Metabolism leukocyte Food Science |
Zdroj: | Wathes, D C, Cheng, Z, Salavati, M, Buggiotti, L, Takeda, H, Tang, L, Becker, F, Ingvartsen, K L, Ferris, C P, Hostens, M, Crowe, M A & GplusE Consortium 2021, ' Relationships between metabolic profiles and gene expression in liver and leukocytes of dairy cows in early lactation ', Journal of Dairy Science, vol. 104, no. 3, pp. 3596-3616 . https://doi.org/10.3168/jds.2020-19165 Wathes, D C, Cheng, Z, Salavati, M, Buggiotti, L, Takeda, H, Tang, L, Becker, F, Ingvartsen, K I, Ferris, C, Hostens, M, Crowe, M A & The GplusE consortium 2021, ' Relationships between metabolic profiles and gene expression in liver and leukocytes of dairy cows in early lactation ', Journal of Dairy Science, vol. 104, no. 3 . https://doi.org/10.3168/jds.2020-19165 |
ISSN: | 1525-3198 |
Popis: | Homeorhetic mechanisms assist dairy cows in the transition from pregnancy to lactation. Less successful cows develop severe negative energy balance (NEB), placing them at risk of metabolic and infectious diseases and reduced fertility. We have previously placed multiparous Holstein Friesian cows from 4 herds into metabolic clusters, using as biomarkers measurements of plasma nonesterified fatty acids, β-hydroxybutyrate, glucose and IGF-1 collected at 14 and 35 d in milk (DIM). This study characterized the global transcriptomic profiles of liver and circulating leukocytes from the same animals to determine underlying mechanisms associated with their metabolic and immune function. Liver biopsy and whole-blood samples were collected around 14 DIM for RNA sequencing. All cows with available RNA sequencing data were placed into balanced (BAL, n = 44), intermediate (n = 44), or imbalanced (IMBAL, n = 19) metabolic cluster groups. Differential gene expression was compared between the 3 groups using ANOVA, but only the comparison between BAL and IMBAL cows is reported. Pathway analysis was undertaken using DAVID Bioinformatic Resources (https://david.ncifcrf.gov/). Milk yields did not differ between BAL and IMBAL cows but dry matter intake was less in IMBAL cows and they were in greater energy deficit at 14 DIM (−4.48 v −11.70 MJ/d for BAL and IMBAL cows). Significantly differentially expressed pathways in hepatic tissue included AMPK signaling, glucagon signaling, adipocytokine signaling, and insulin resistance. Genes involved in lipid metabolism and cholesterol transport were more highly expressed in IMBAL cows but IGF1 and IGFALS were downregulated. Leukocytes from BAL cows had greater expression of histones and genes involved in nucleosomes and cell division. Leukocyte expression of heat shock proteins increased in IMBAL cows, suggesting an unfolded protein response, and several key genes involved in immune responses to pathogens were upregulated (e.g., DEFB13, HP, OAS1Z, PTX3, and TLR4). Differentially expressed genes upregulated in IMBAL cows in both tissues included CD36, CPT1, KFL11, and PDK4, all central regulators of energy metabolism. The IMBAL cows therefore had greater difficulty maintaining glucose homeostasis and had dysregulated hepatic lipid metabolism. Their energy deficit was associated with a reduced capacity for cell division and greater evidence of stress responses in the leukocyte population, likely contributing to an increased risk of infectious disease. |
Databáze: | OpenAIRE |
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