Radiation Effects on Osteoblasts In Vitro
| Autor: | Douglas W. Klotch, Teresita Muñoz-Antonia, T. J. Gal, Carlos A. Muro-Cacho |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Pathology
medicine.medical_specialty Osteoradionecrosis Receptor expression Dexamethasone Bone remodeling Mice medicine Animals Prospective Studies Receptor Glucocorticoids Cells Cultured Osteoblasts business.industry Cell growth Osteoblast General Medicine medicine.disease Immunohistochemistry medicine.anatomical_structure Otorhinolaryngology Cancer research Surgery Bone Remodeling Collagen Bone Diseases business Wound healing Receptors Transforming Growth Factor beta Transforming growth factor |
| Zdroj: | Archives of Otolaryngology–Head & Neck Surgery. 126:1124 |
| ISSN: | 0886-4470 |
| DOI: | 10.1001/archotol.126.9.1124 |
| Popis: | Objective To evaluate the factors involved in bone remodeling and wound healing that may be altered by radiation therapy. Design A prospective, controlled study of biochemical activity in vitro. Subjects MC3T3-E1 mouse osteoblasts. Interventions Cells were irradiated at 0, 2, 4, or 6 Gy. Specimens were harvested at 1, 7, 14, 28, and 42 days following irradiation for immunohistochemical analysis of transforming growth factor β 1 expression and transforming growth factor β 1 type I and II receptor expression. Collagen production was measured at 1, 7, 28, 35, and 49 days after irradiation. The effects of dexamethasone on collagen production and cell proliferation were also examined. Results Irradiated cells demonstrated decreased cell proliferation and a dose-dependent, sustained reduction in collagen production when compared with control cells. An increase in transforming growth factor β 1 type I and II receptor expression was noted in irradiated cells when compared with controls. Conclusion Radiation-induced alterations of factors related to bone remodeling and wound healing have a potential role in the pathogenesis of osteoradionecrosis. |
| Databáze: | OpenAIRE |
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