Circadian Analysis of the Mouse Cerebellum Proteome
| Autor: | Marie-Paule Felder-Schmittbuhl, Fabrice Bertile, Marine Plumel, Cristina Sandu, Stéphanie Dumont, Pauline Maes, Etienne Challet |
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| Přispěvatelé: | Institut Pluridisciplinaire Hubert Curien (IPHC), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Institut des Neurosciences Cellulaires et Intégratives (INCI), Département Sciences Analytiques et Interactions Ioniques et Biomoléculaires (DSA-IPHC), Université de Strasbourg (UNISTRA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Male
Proteomics Cerebellum Proteome Circadian clock clock gene Aucun lcsh:Chemistry Two-Dimensional Difference Gel Electrophoresis Mice 0302 clinical medicine bmal1 lcsh:QH301-705.5 Spectroscopy Gel electrophoresis 0303 health sciences carbonic-anhydrase General Medicine Computer Science Applications Cell biology CLOCK period Chemistry medicine.anatomical_structure oscillations 2D-DIGE/MS 2D-DIGE transcription roles circadian rhythm Biochemistry & Molecular Biology cerebellum rev-erb Biology Catalysis Article Inorganic Chemistry 03 medical and health sciences suprachiasmatic nuclei Glutamine synthetase Circadian Clocks medicine Animals [CHIM]Chemical Sciences Synapsin 2 Circadian rhythm RNA Messenger Physical and Theoretical Chemistry Molecular Biology mouse 030304 developmental biology Organic Chemistry MS Mice Inbred C57BL lcsh:Biology (General) lcsh:QD1-999 Gene Expression Regulation 030217 neurology & neurosurgery |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, MDPI, 2019, 20 (8), pp.1852. ⟨10.3390/ijms20081852⟩ International Journal of Molecular Sciences, Vol 20, Iss 8, p 1852 (2019) Volume 20 Issue 8 |
| ISSN: | 1422-0067 1661-6596 |
| DOI: | 10.3390/ijms20081852⟩ |
| Popis: | International audience; The cerebellum contains a circadian clock, generating internal temporal signals. The daily oscillations of cerebellar proteins were investigated in mice using a large-scale two-dimensional difference in gel electrophoresis (2D-DIGE). Analysis of 2D-DIGE gels highlighted the rhythmic variation in the intensity of 27/588 protein spots (5%) over 24 h based on cosinor regression. Notably, the rhythmic expression of most abundant cerebellar proteins was clustered in two main phases (i.e., midday and midnight), leading to bimodal distribution. Only six proteins identified here to be rhythmic in the cerebellum are also known to oscillate in the suprachiasmatic nuclei, including two proteins involved in the synapse activity (Synapsin 2 [SYN2] and vesicle-fusing ATPase [NSF]), two others participating in carbohydrate metabolism (triosephosphate isomerase (TPI1] and alpha-enolase [ENO1]), Glutamine synthetase (GLUL), as well as Tubulin alpha (TUBA4A). Most oscillating cerebellar proteins were not previously identified in circadian proteomic analyses of any tissue. Strikingly, the daily accumulation of mitochondrial proteins was clustered to the mid-resting phase, as previously observed for distinct mitochondrial proteins in the liver. Moreover, a number of rhythmic proteins, such as SYN2, NSF and TPI1, were associated with non-rhythmic mRNAs, indicating widespread post-transcriptional control in cerebellar oscillations. Thus, this study highlights extensive rhythmic aspects of the cerebellar proteome. |
| Databáze: | OpenAIRE |
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