Continuous Packed Bed Reactor with Immobilized β-Galactosidase for Production of Galactooligosaccharides (GOS)
| Autor: | Lucia Fernandez-Arrojo, Paloma Santos-Moriano, Barbara Rodriguez-Colinas, Francisco J. Plou, Antonio Ballesteros |
|---|---|
| Přispěvatelé: | Ministerio de Economía y Competitividad (España), Consejo Superior de Investigaciones Científicas (España) |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
fixed-bed reactor Batch reactor lcsh:Chemical technology 01 natural sciences Catalysis Continuous production lcsh:Chemistry chemistry.chemical_compound 010608 biotechnology Bioreactor galacto-oligosaccharides lcsh:TP1-1185 covalent immobilization Physical and Theoretical Chemistry Lactose glyoxal agarose Packed bed Chromatography 010405 organic chemistry betα-galactosidase 0104 chemical sciences chemistry lcsh:QD1-999 Bacillus circulans Agarose Glyoxal prebiotics transglycosylation |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname Catalysts; Volume 6; Issue 12; Pages: 189 Catalysts, Vol 6, Iss 12, p 189 (2016) |
| Popis: | The β-galactosidase from Bacillus circulans was covalently attached to aldehyde-activated (glyoxal) agarose beads and assayed for the continuous production of galactooligosaccharides (GOS) in a packed-bed reactor (PBR). The immobilization was fast (1 h) and the activity of the resulting biocatalyst was 97.4 U/g measured with o-nitrophenyl-β-d-galactopyranoside (ONPG). The biocatalyst showed excellent operational stability in 14 successive 20 min reaction cycles at 45 °C in a batch reactor. A continuous process for GOS synthesis was operated for 213 h at 0.2 mL/min and 45 °C using 100 g/L of lactose as a feed solution. The efficiency of the PBR slightly decreased with time; however, the maximum GOS concentration (24.2 g/L) was obtained after 48 h of operation, which corresponded to 48.6% lactose conversion and thus to maximum transgalactosylation activity. HPAEC-PAD analysis showed that the two major GOS were the trisaccharide Gal-β(1→4)-Gal-β(1→4)-Glc and the tetrasaccharide Gal-β(1→4)-Gal-β(1→4)-Gal-β(1→4)-Glc. The PBR was also assessed in the production of GOS from milk as a feed solution. The stability of the bioreactor was satisfactory during the first 8 h of operation; after that, a decrease in the flow rate was observed, probably due to partial clogging of the column. This work represents a step forward in the continuous production of GOS employing fixed-bed reactors with immobilized β-galactosidases. This work was supported by grants from the Spanish Ministry of Economy and Competitiveness (BIO2013-48779-C4-1-R and BIO2016-76601-C3-1-R). We thank the support of COST-Action CM1303 on Systems Biocatalysis. P.S.-M. thanks the Spanish Ministry of Education for FPU Grant (FPU13/01185). We acknowledge support by the CSIC Open Access Publication Initiative through its Unit of Information Resources for Research (URICI). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|