Microchip-based ultrafast serodiagnostic assay for tuberculosis
| Autor: | Jo Ann Tay, Pavanish Kumar, Amit Singhal, Karima Larbi, Bhairav Paleja, Jie Yee Siew, Utkan Demirci, ShuQi Wang, Gennaro De Libero, Vigneshwaran Mani, Yee Tang Wang, Cynthia B.E. Chee, Fatih Inci |
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| Rok vydání: | 2016 |
| Předmět: |
Multidisciplinary
Tuberculosis Receiver operating characteristic analysis Latent tuberculosis Plasma samples business.industry 010401 analytical chemistry 02 engineering and technology 021001 nanoscience & nanotechnology medicine.disease Active tuberculosis 01 natural sciences Article 0104 chemical sciences Mycobacterial antigen Active tb Immunology medicine Sputum medicine.symptom 0210 nano-technology business |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Access to point-of-care (POC), rapid, inexpensive, sensitive, and instrument-free tests for the diagnosis of tuberculosis (TB) remains a major challenge. Here, we report a simple and low-cost microchip-based TB ELISA (MTBE) platform for the detection of anti-mycobacterial IgG in plasma samples in less than 15 minutes. The MTBE employs a flow-less, magnet-actuated, bead-based ELISA for simultaneous detection of IgG responses against multiple mycobacterial antigens. Anti-trehalose 6,6′-dimycolate (TDM) IgG responses were the strongest predictor for differentiating active tuberculosis (ATB) from healthy controls (HC) and latent tuberculosis infections (LTBI). The TDM-based MTBE demonstrated superior sensitivity compared to sputum microscopy (72% vs. 56%) with 80% and 63% positivity among smear-positive and smear-negative confirmed ATB samples, respectively. Receiver operating characteristic analysis indicated good accuracy for differentiating ATB from HC (AUC = 0.77). Thus, TDM-based MTBE can be potentially used as a screening device for rapid diagnosis of active TB at the POC. |
| Databáze: | OpenAIRE |
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