Interferon production and tumor cell killing by human lymphocytes stimulated in mixed-lymphocyte culture
| Autor: | Peterman Gm, Stanton Gj, Klimpel Gr, Altman Lc |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
CD40
Lymphokine-activated killer cell T-Lymphocytes T cell Immunology Antibodies Monoclonal chemical and pharmacologic phenomena Biology Natural killer T cell Molecular biology Killer Cells Natural Kinetics Interleukin 21 medicine.anatomical_structure medicine biology.protein Interleukin 12 Humans Cytotoxic T cell Interferons Lymphocytes Lymphocyte Culture Test Mixed Cytotoxicity |
| Zdroj: | Cellular Immunology. 85:114-124 |
| ISSN: | 0008-8749 |
| DOI: | 10.1016/0008-8749(84)90283-1 |
| Popis: | The in vitro synthesis of interferon (IFN) by human lymphocytes stimulated in mixed-lymphocyte culture (MLC) was examined. The production of IFN in MLC was restricted to T lymphocytes and maximum levels of IFN were detected in supernatants from cells incubated for 5 to 7 days. The IFN produced was identified as IFN-gamma by antibody neutralization. To identify the T cell responsible for IFN production, purified T lymphocytes were separated into subpopulations after incubation in 5 mM theophylline. Theophylline-resistant (T-res) T cells retain the ability to form sheep erythrocyte (SRBC) rosettes and are depleted in IgG Fc receptor-positive T cells (T gamma cells). Theophylline-sensitive (T-sens) T cells fail to form rosettes after theophylline treatment and are enriched in T gamma cells. In addition, analyses using monoclonal antibodies showed that T-sens cells were enriched in OKM1-, HNK-1-, and 7.2-positive cells and T-res cells contained increased numbers of 9.6- and OKT4-positive cells. Following MLC stimulation, equivalent levels of IFN-gamma were produced by T-res and T-sens cells and both subpopulations maintained natural killer (NK)-like cytotoxicity against K562 target cells. Addition of partially purified IFN-gamma to unstimulated T-res and T-sens cells resulted in the maintenance of NK-like cytotoxicity in a manner analogous to that observed after MLC. Additional experiments indicated that peripheral blood lymphocytes depleted of 9.6- or OKM1-positive cells by complement-mediated lysis were devoid of cytotoxicity against K562 cells. Furthermore, MLC stimulation of 9.6- or OKM1-depleted cells failed to restore cytotoxic activity. In summary, these experiments demonstrate that the maintenance of NK-like cytotoxicity by MLC-stimulated T cells is associated with the synthesis of IFN-gamma, that MLC stimulated T-res and T-sens T-cell subsets produce equivalent amounts of IFN, and that 9.6- or OKM1-positive cells are required for the maintenance of NK-like cytotoxicity in MLC. |
| Databáze: | OpenAIRE |
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