Using sweeping micellar electrokinetic chromatography to analyze Δ9-tetrahydrocannabinol and its major metabolites
| Autor: | Hsiu Li Su, Hsiu Ping Jen, You-Zung Hsieh, Lan Ing Feng |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Detection limit
Chromatography Chemistry Methanol Metabolite Solid Phase Extraction Organic Chemistry General Medicine Reference Standards Biochemistry Micellar electrokinetic chromatography Analytical Chemistry chemistry.chemical_compound Calibration Delta-9-tetrahydrocannabinol Humans Sample preparation Dronabinol Solid phase extraction Sodium dodecyl sulfate Quantitative analysis (chemistry) Chromatography Micellar Electrokinetic Capillary |
| Zdroj: | Journal of Chromatography A. 1216:3512-3517 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/j.chroma.2008.10.004 |
| Popis: | We have applied sweeping micellar electrokinetic chromatography (sweeping-MEKC) to the simultaneous determination of Delta(9)-tetrahydrocannabinol (THC) and its major metabolites, 11-hydroxy-Delta(9)-tetrahydrocannabinol (THC-OH) and 11-nor-9-carboxy-Delta(9)-tetrahydrocannabinol (THC-COOH). We monitored the effects of several of the sweeping-MEKC parameters, including the proportion of organic modifier, the concentration of sodium dodecyl sulfate (SDS), the pH, and the sample injection volume, to optimize the separation process. The optimal buffer for the analysis of the three analytes was 25 mM citric acid/disodium hydrogenphosphate (pH 2.6) containing 40% methanol and 75 mM SDS. Under the optimized separation parameters, the enrichment factors for THC, THC-COOH, and THC-OH when using sweeping-MEKC (relative to MEKC) were 77, 139, and 200, respectively. The limits of detection (LODs) for the three compounds in standard solutions ranged from 3.87 to 15.2 ng/mL. We combined the sweeping-MEKC method with solid-phase extraction to successfully detect THC, THC-COOH, and THC-OH in human urine with acceptable repeatability. The LODs of these analytes in urine samples ranged from 17.2 to 23.3 ng/mL. Therefore, this sweeping-MEKC method is useful for determining, with high sensitivity, the amounts of THC and its metabolites in the urine of suspected THC users. |
| Databáze: | OpenAIRE |
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