A top‐down systems biology view of microbiome‐mammalian metabolic interactions in a mouse model

Autor: Olivier Cloarec, Cristina Legido-Quigley, François-Pierre Martin, Jeremy K. Nicholson, John C. Lindon, Yulan Wang, Huiru Tang, Gerard M. Murphy, Sunil Kochhar, Ivan K. S. Yap, Elaine Holmes, Marc-Emmanuel Dumas, Severine Zirah, Laurent B. Fay, Peter J. van Bladeren, Norbert Sprenger
Přispěvatelé: Biomolecular Medicine, Department of Surgery and Cancer Faculty of Medicine, Imperial College London, Nestle Research Center, Nestlé Research Center, Department of Surgery & Cancer, Chinese Academy of Sciences (CAS)
Jazyk: angličtina
Rok vydání: 2007
Předmět:
metabonomics/metabolomics
Systems biology
gut microflora
Ileum
Biology
01 natural sciences
Article
General Biochemistry
Genetics and Molecular Biology
Microbiology
Mice
03 medical and health sciences
Cecum
chemistry.chemical_compound
[CHIM.ANAL]Chemical Sciences/Analytical chemistry
medicine
Metabolome
Animals
Humans
Microbiome
Feces
ComputingMilieux_MISCELLANEOUS
030304 developmental biology
network modeling
2. Zero hunger
0303 health sciences
General Immunology and Microbiology
Systems Biology
Applied Mathematics
010401 analytical chemistry
Glutathione
Metabolism
[SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism
0104 chemical sciences
Gastrointestinal Tract
medicine.anatomical_structure
Computational Theory and Mathematics
Biochemistry
chemistry
Organ Specificity
co-metabolism
General Agricultural and Biological Sciences
Information Systems
[SDV.EE.IEO]Life Sciences [q-bio]/Ecology
environment/Symbiosis
Zdroj: Molecular Systems Biology
Molecular Systems Biology, EMBO Press, 2007, 3 (1), pp.112. ⟨10.1038/msb4100153⟩
ISSN: 1744-4292
Popis: Symbiotic gut microorganisms (microbiome) interact closely with the mammalian host's metabolism and are important determinants of human health. Here, we decipher the complex metabolic effects of microbial manipulation, by comparing germfree mice colonized by a human baby flora (HBF) or a normal flora to conventional mice. We perform parallel microbiological profiling, metabolic profiling by 1H nuclear magnetic resonance of liver, plasma, urine and ileal flushes, and targeted profiling of bile acids by ultra performance liquid chromatography–mass spectrometry and short-chain fatty acids in cecum by GC-FID. Top-down multivariate analysis of metabolic profiles reveals a significant association of specific metabotypes with the resident microbiome. We derive a transgenomic graph model showing that HBF flora has a remarkably simple microbiome/metabolome correlation network, impacting directly on the host's ability to metabolize lipids: HBF mice present higher ileal concentrations of tauro-conjugated bile acids, reduced plasma levels of lipoproteins but higher hepatic triglyceride content associated with depletion of glutathione. These data indicate that the microbiome modulates absorption, storage and the energy harvest from the diet at the systems level.
Databáze: OpenAIRE
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