A top‐down systems biology view of microbiome‐mammalian metabolic interactions in a mouse model
Autor: | Olivier Cloarec, Cristina Legido-Quigley, François-Pierre Martin, Jeremy K. Nicholson, John C. Lindon, Yulan Wang, Huiru Tang, Gerard M. Murphy, Sunil Kochhar, Ivan K. S. Yap, Elaine Holmes, Marc-Emmanuel Dumas, Severine Zirah, Laurent B. Fay, Peter J. van Bladeren, Norbert Sprenger |
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Přispěvatelé: | Biomolecular Medicine, Department of Surgery and Cancer Faculty of Medicine, Imperial College London, Nestle Research Center, Nestlé Research Center, Department of Surgery & Cancer, Chinese Academy of Sciences (CAS) |
Jazyk: | angličtina |
Rok vydání: | 2007 |
Předmět: |
metabonomics/metabolomics
Systems biology gut microflora Ileum Biology 01 natural sciences Article General Biochemistry Genetics and Molecular Biology Microbiology Mice 03 medical and health sciences Cecum chemistry.chemical_compound [CHIM.ANAL]Chemical Sciences/Analytical chemistry medicine Metabolome Animals Humans Microbiome Feces ComputingMilieux_MISCELLANEOUS 030304 developmental biology network modeling 2. Zero hunger 0303 health sciences General Immunology and Microbiology Systems Biology Applied Mathematics 010401 analytical chemistry Glutathione Metabolism [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism 0104 chemical sciences Gastrointestinal Tract medicine.anatomical_structure Computational Theory and Mathematics Biochemistry chemistry Organ Specificity co-metabolism General Agricultural and Biological Sciences Information Systems [SDV.EE.IEO]Life Sciences [q-bio]/Ecology environment/Symbiosis |
Zdroj: | Molecular Systems Biology Molecular Systems Biology, EMBO Press, 2007, 3 (1), pp.112. ⟨10.1038/msb4100153⟩ |
ISSN: | 1744-4292 |
Popis: | Symbiotic gut microorganisms (microbiome) interact closely with the mammalian host's metabolism and are important determinants of human health. Here, we decipher the complex metabolic effects of microbial manipulation, by comparing germfree mice colonized by a human baby flora (HBF) or a normal flora to conventional mice. We perform parallel microbiological profiling, metabolic profiling by 1H nuclear magnetic resonance of liver, plasma, urine and ileal flushes, and targeted profiling of bile acids by ultra performance liquid chromatography–mass spectrometry and short-chain fatty acids in cecum by GC-FID. Top-down multivariate analysis of metabolic profiles reveals a significant association of specific metabotypes with the resident microbiome. We derive a transgenomic graph model showing that HBF flora has a remarkably simple microbiome/metabolome correlation network, impacting directly on the host's ability to metabolize lipids: HBF mice present higher ileal concentrations of tauro-conjugated bile acids, reduced plasma levels of lipoproteins but higher hepatic triglyceride content associated with depletion of glutathione. These data indicate that the microbiome modulates absorption, storage and the energy harvest from the diet at the systems level. |
Databáze: | OpenAIRE |
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