Stability of a dominant sponge‐symbiont in spite of antibiotic‐induced microbiome disturbance

Autor: Lara Schmittmann, Tanja Rahn, Kathrin Busch, Sebastian Fraune, Lucía Pita, Ute Hentschel
Přispěvatelé: Gordon and Betty Moore Foundation, German Research Foundation, International Max Planck Research Schools, Fundación 'la Caixa', European Commission, Agencia Estatal de Investigación (España)
Rok vydání: 2022
Předmět:
Zdroj: Environmental Microbiology. 24:6392-6410
ISSN: 1462-2920
1462-2912
Popis: 19 pages, 4 figures, 1 table, supporting information https://doi.org/10.1111/1462-2920.16249.-- Data Availability Statement: All 16 S rRNA gene amplicon reads and the sample metadata and attributes are available in the National Center for Biotechnology Information (NCBI) Sequence Read Archive (SRA): PRJNA786895; BioSample accessions: SAMN23732322- SAMN23732615
Marine sponges are known for their complex and stable microbiomes. However, the lack of a gnotobiotic sponge-model and experimental methods to manipulate both the host and the microbial symbionts currently limit our mechanistic understanding of sponge-microbial symbioses. We have used the North Atlantic sponge species Halichondria panicea to evaluate the use of antibiotics to generate gnotobiotic sponges. We further asked whether the microbiome can be reestablished via recolonization with the natural microbiome. Experiments were performed in marine gnotobiotic facilities equipped with a custom-made, sterile, flow-through aquarium system. Bacterial abundance dynamics were monitored qualitatively and quantitatively by 16 S rRNA gene amplicon sequencing and qPCR, respectively. Antibiotics induced dysbiosis by favouring an increase of opportunistic, antibiotic-resistant bacteria, resulting in more complex, but less specific bacteria-bacteria interactions than in untreated sponges. The abundance of the dominant symbiont, Candidatus Halichondribacter symbioticus, remained overall unchanged, reflecting its obligately symbiotic nature. Recolonization with the natural microbiome could not reverse antibiotic-induced dysbiosis. However, single bacterial taxa that were transferred, successfully recolonized the sponge and affected bacteria-bacteria interactions. By experimentally manipulating microbiome composition, we could show the stability of a sponge-symbiont clade despite microbiome dysbiosis. This study contributes to understanding both host-bacteria and bacteria-bacteria interactions in the sponge holobiont
This project is supported by funding of the DFG (“Origin and Function of Metaorganisms”, CRC1182-TP B01) and the Gordon and Betty Moore Foundation (“Symbiosis in Aquatic Systems Initiative”, GBMF9352) to UH. LS is further supported by the International Max Planck Research School for Evolutionary Biology, and LP receives funding by the DFG (‘IMMUBASE’, 417,981,041) and is currently supported by ‘la Caixa’ Foundation (ID 10010434) and from the European Union's Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grand agreement No 847648, fellowship code is 104855
With the institutional support of the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S)
Databáze: OpenAIRE
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