The relationship between crosslinking structure and silk fibroin scaffold performance for soft tissue engineering
| Autor: | Juan Guan, Lei Sun, Xiong Shu, Xiaoming Li, Sujun Wu, Zhinan Mao, Puyu Du, Xuewei Bi, Fan Ye |
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| Rok vydání: | 2021 |
| Předmět: |
Scaffold
Fibroin 02 engineering and technology Biochemistry Rats Sprague-Dawley Mice 03 medical and health sciences Structural Biology In vivo Soft tissue engineering Animals Molecular Biology Mechanical Phenomena 030304 developmental biology Ethylene glycol diglycidyl ether 0303 health sciences Cell Death Tissue Engineering Tissue Scaffolds Chemistry Foreign-Body Reaction Water Biological activity General Medicine Bombyx 021001 nanoscience & nanotechnology In vitro Cross-Linking Reagents NIH 3T3 Cells Subcutaneous implantation Biophysics Fibroins 0210 nano-technology |
| Zdroj: | International Journal of Biological Macromolecules. 182:1268-1277 |
| ISSN: | 0141-8130 |
| Popis: | Biologically active scaffolds with tunable mechano- and bio-performance remain desirable for soft tissue engineering. Previously, highly elastic and robust silk fibroin (SF) scaffolds were prepared via cryogelation. In order to get more insight into the role of ethylene glycol diglycidyl ether (EGDE) on the structure and properties of SF scaffolds, we investigated the fate of SF scaffolds with different usages of the crosslinking agent in vitro and in vivo. Although SF scaffolds with varied EGDE contents showed similar micro-morphology, increasing EGDE from 1 mmol/g to 5 mmol/g resulted in firstly increased and later decreased content of β-sheet conformation, and linearly increased tensile modulus and decreased elasticity. The dual-crosslinked SF scaffolds with EGDE up to 5 mmol/g did not show in vitro cytotoxicity for NIH3T3 fibroblasts. In vivo subcutaneous implantation of SF scaffolds with |
| Databáze: | OpenAIRE |
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