Characterization of the first tetrameric transcription factor of the GntR superfamily with allosteric regulation from the bacterial pathogen Agrobacterium fabrum
| Autor: | Armelle Vigouroux, Anaïs Naretto, Pierre Legrand, Aurélie Di Cicco, Ludovic Vial, Daniel Lévy, Sébastien Renoud, Céline Lavire, Jeanne Doré, Magali Aumont-Nicaise, Solange Moréra, Thibault Meyer |
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| Přispěvatelé: | Centre National de la Recherche Scientifique (CNRS), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Synchroton Soleil, Division Experience/Ligne Nanoscopium, L'Orme des Merisiers, Gif-sur-Yvette, ANR-10-INSB-05-01 INSB ANR-10-INSB-0501, Universite de LyonED341-E2M2French Ministere de l'Education Nationale, de l'Enseignement Superieur et de la RechercheFrench National Research Infrastructure France BioImagingANR10-INBS-04Centre National de la Recherche Scientifique (CNRS)European Commission, Synchrotron SOLEIL (SSOLEIL), Laboratoire Physico-Chimie Curie [Institut Curie] (PCC), Institut Curie [Paris]-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Microbiologie et enzymologie structurale (MESB3S), Département Biochimie, Biophysique et Biologie Structurale (B3S), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Mesures d'interactions protéine-protéine (PIM), Département Plateforme (PF I2BC), Centre de recherche de l'Institut Curie [Paris], Institut Curie [Paris] |
| Rok vydání: | 2020 |
| Předmět: |
Models
Molecular Coumaric Acids Protein Conformation AcademicSubjects/SCI00010 [SDV]Life Sciences [q-bio] Allosteric regulation Repressor Agrobacterium Biology Crystallography X-Ray Sodium Citrate 03 medical and health sciences Allosteric Regulation Bacterial Proteins Protein Domains Transcription (biology) Structural Biology Genetics Transcriptional regulation Genes Synthetic Binding site Promoter Regions Genetic Transcription factor Tetrahydrofolates 030304 developmental biology 0303 health sciences Binding Sites Effector 030302 biochemistry & molecular biology Gene Expression Regulation Bacterial DNA binding site Repressor Proteins Zinc Biochemistry Multigene Family Protein Multimerization |
| Zdroj: | Nucleic Acids Research Nucleic Acids Research, Oxford University Press, 2021, 49 (1), pp.529-546. ⟨10.1093/nar/gkaa1181⟩ Nucleic Acids Research, 2021, 49 (1), pp.529-546. ⟨10.1093/nar/gkaa1181⟩ Nucleic Acids Research, 2020, ⟨10.1093/nar/gkaa1181⟩ |
| ISSN: | 1362-4962 0305-1048 |
| Popis: | A species-specific region, denoted SpG8-1b allowing hydroxycinnamic acids (HCAs) degradation is important for the transition between the two lifestyles (rhizospheric versus pathogenic) of the plant pathogen Agrobacterium fabrum. Indeed, HCAs can be either used as trophic resources and/or as induced-virulence molecules. The SpG8-1b region is regulated by two transcriptional regulators, namely, HcaR (Atu1422) and Atu1419. In contrast to HcaR, Atu1419 remains so far uncharacterized. The high-resolution crystal structures of two fortuitous citrate complexes, two DNA complexes and the apoform revealed that the tetrameric Atu1419 transcriptional regulator belongs to the VanR group of Pfam PF07729 subfamily of the large GntR superfamily. Until now, GntR regulators were described as dimers. Here, we showed that Atu1419 represses three genes of the HCAs catabolic pathway. We characterized both the effector and DNA binding sites and identified key nucleotides in the target palindrome. From promoter activity measurement using defective gene mutants, structural analysis and gel-shift assays, we propose N5,N10-methylenetetrahydrofolate as the effector molecule, which is not a direct product/substrate of the HCA degradation pathway. The Zn2+ ion present in the effector domain has both a structural and regulatory role. Overall, our work shed light on the allosteric mechanism of transcription employed by this GntR repressor. |
| Databáze: | OpenAIRE |
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