Developmental expression of choline acetyltransferase mRNA in Drosophila
| Autor: | Paul M. Salvaterra, L. A. Carbini, V. J. Munoz Maines |
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| Rok vydání: | 1990 |
| Předmět: |
Blotting
Western Biology Biochemistry Gene Expression Regulation Enzymologic Choline O-Acetyltransferase Cellular and Molecular Neuroscience Drosophilidae Gene expression Melanogaster Animals RNA Messenger Genetics Messenger RNA fungi RNA Nucleic Acid Hybridization General Medicine RNA Probes biology.organism_classification Blotting Northern Molecular biology Choline acetyltransferase Drosophila melanogaster Cell culture Larva DNA Probes |
| Zdroj: | Neurochemical research. 15(11) |
| ISSN: | 0364-3190 |
| Popis: | We have measured the steady state levels of choline acetyltransferase (ChAT, EC 2.3.1.6) mRNA during different developmental stages of Drosophila melanogaster using a ChAT specific cRNA probe. ChAT mRNA was first detected approximately 6-7 h after oviposition, increased until the 1st-2nd larval instar, decreased into early pupal stages and increased again during late pupation, reaching a maximum in adults. Northern analysis showed a major RNA band with a Mr of 4.7 kilobases and Western analysis also showed a single major 75 kD protein band at all developmental stages. Our results support the hypothesis that a major point of regulation of ChAT expression may be at the transcriptional level. |
| Databáze: | OpenAIRE |
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