A new approach to obtain cellulose nanocrystals and ethanol from eucalyptus cellulose pulp via the biochemical pathway
Autor: | Thalita J. Bondancia, Cristiane S. Farinas, Luiz H. C. Mattoso, José Manoel Marconcini |
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Přispěvatelé: | LUIZ HENRIQUE CAPPARELLI MATTOSO, CNPDIA, JOSE MANOEL MARCONCINI, CNPDIA, CRISTIANE SANCHEZ FARINAS, CNPDIA. |
Rok vydání: | 2017 |
Předmět: |
Enzimatic hydrolysis
Central composite design Saccharomyces cerevisiae 02 engineering and technology Renewable biomass Raw material 01 natural sciences chemistry.chemical_compound Crystallinity Enzymatic hydrolysis Cellulases Cellulose Cellulose nanocrystal Eucalyptus Ethanol 010405 organic chemistry business.industry Hydrolysis 021001 nanoscience & nanotechnology Biorefinery 0104 chemical sciences Biotechnology chemistry Chemical engineering Cellulosic ethanol Yield (chemistry) Nanoparticles 0210 nano-technology business |
Zdroj: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
ISSN: | 8756-7938 |
Popis: | The feasibility of integration of cellulosic ethanol production with the manufacture of cellulose nanofibers (CNF) and cellulose nanocrystals (CNC) was evaluated using eucalyptus cellulose pulp as feedstock and employing the biochemical route alone. For the enzymatic hydrolysis step, experimental central composite design (CCD) methodology was used as a tool to evaluate the effects of solids loading (SL) and enzymatic loading (EL) on glucose release and cellulose conversion. Glucose concentrations from 45 to 125 g/L were obtained after 24 h, with cellulose conversions from 35 to 96%. Validation of the statistical model was performed at SL of 20% and EL of 10 mg protein/g, which was defined by the desirability function as the optimum condition. The sugars released were used for the production of ethanol by Saccharomyces cerevisiae, resulting in 62.1 g/L ethanol after 8 h (yield of 95.5%). For all the CCD experimental conditions, the residual solids presented CNF characteristics. Moreover, the use of a new strategy with temperature reduction from 50 to 35°C after 24 h of enzymatic hydrolysis enabled CNC to be obtained after 144 h. The CNC showed a crystallinity index of 83%, length of 260 nm, diameter of 15 nm, and aspect ratio (L/D) of 15. These characteristics are suitable for many applications, such as reinforcement in polymeric materials and other lower volume higher value bio-based products. The findings indicate the viability of obtaining ethanol and CNC using the biochemical route exclusively, potentially contributing to the future implementation of forest biorefineries. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:1085-1095, 2017. |
Databáze: | OpenAIRE |
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