Screening prolyl hydroxylase domain 2 inhibitory activity of traditional Chinese medicine by CZE‐UV

Autor:	Ying Zhang, Ming Zhao, Cheng‐Bo Wang, Yu Wang, Stanislas Nsanzamahoro, Ling‐Ling Zhu, Wei‐Feng Wang, Jun‐Li Yang
Rok vydání:	2022
Předmět:	Clinical Biochemistry Procollagen-Proline Dioxygenase Electrophoresis Capillary Humans Medicine Chinese Traditional Hypoxia Hypoxia-Inducible Factor 1 alpha Subunit Biochemistry Hypoxia-Inducible Factor-Proline Dioxygenases Analytical Chemistry
Zdroj:	ELECTROPHORESIS. 43:1601-1610
ISSN:	1522-2683 0173-0835
Popis:	Prolyl hydroxylase domain 2 (PHD2) is a key enzyme regulating the expression of hypoxia inducible factor (HIF). Its inhibitors can improve the expression of HIF and downstream genes, which can treat hypoxia-related diseases. Therefore, the establishment of a reliable PHD2 inhibitors screening method is of great significance for the drug development of hypoxia-related diseases. In this work, an accurate, rapid, and simple screening method for PHD2 inhibitors was introduced by capillary zone electrophoresis (CZE). In order to improve the detection sensitivity, the derivative reaction of α-ketoglutaric acid (α-OG) and 1,2-diaminobenzene (OPD) was used to enhance the UV absorption of α-OG (the substrate in the enzymatic reaction). The CZE method selected 20 mM Na
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8076854983f3ae6bbdaa3462c29bbadd https://doi.org/10.1002/elps.202200028 Zobrazit plný text záznamu Plný text