Preliminary crystallographic studies of a Schistosoma mansoni antigen (Sm21.7) dynein light-chain (DLC) domain
| Autor: | F. T. G. Rodrigues, Ronaldo Alves Pinto Nagem, Mariana Amalia Figueiredo Costa, B. C. A. Chagas, Alfredo M. Goes, Cíntia M.F. Rezende |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Biophysics
Schistosomiasis Antigens Protozoan medicine.disease_cause Immunoglobulin light chain Crystallography X-Ray Biochemistry Epitope law.invention Antigen Structural Biology law parasitic diseases Genetics medicine Animals Escherichia coli Gene DNA Primers biology Base Sequence Schistosoma mansoni biochemical phenomena metabolism and nutrition Condensed Matter Physics biology.organism_classification medicine.disease Crystallography Crystallization Communications Recombinant DNA bacteria |
| Popis: | Schistosomiasis is an inflammatory chronic disease that represents a major health problem in tropical and subtropical countries. The drug of choice for treatment, praziquantel, is effective in killing adult worms but fails to kill immature forms and prevent reinfection. One prominent antigen candidate for an anti-schistosomiasis vaccine is the protein Sm21.7 (184 amino-acid residues) fromSchistosoma mansoni, a tegumental protein capable of reducing the worm burden in a murine immunization model. In the present work, the Sm21.7 gene was cloned and expressed inEscherichia coliand the full-length protein was purified to homogeneity. Crystals of recombinant Sm21.7 suitable for X-ray diffraction were obtained using PEG monomethyl ether 2000 as a precipitant. X-ray diffraction images of a native crystal (at 2.05 Å resolution) and a quick-cryosoaked NaI derivative (at 1.95 Å resolution) were collected on the W01B-MX2 beamline at the Laboratório Nacional de Luz Síncrotron (LNLS, Brazilian Synchrotron Light Laboratory/MCT). Both crystals belonged to the hexagonal space groupP6122, with similar unit-cell parametersa=b= 108.5,c= 55.8 Å. SIRAS-derived phases were used to generate the first electron-density map, from which a partial three-dimensional model of Sm21.7 (from Gln89 to Asn184) was automatically constructed. Anaysis of dissolved crystals by SDS–PAGE confirmed that the protein was cleaved in the crystallization drop and only the Sm21.7 C-terminal domain was crystallized. The structure of the Sm21.7 C-terminal domain will help in the localization of the epitopes responsible for its protective immune responses, constituting important progress in the development of an anti-schistosomiasis vaccine. |
| Databáze: | OpenAIRE |
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