ER-MP12 antigen, a new cell surface marker on mouse bone marrow cells with thymus-repopulating ability: I. Intrathymic repopulating ability of ER-MP12-positive bone marrow cells
| Autor: | Walentina A. T. Slieker, M. F. T. R. De Bruijn, W. Van Ewijk, Pieter J. M. Leenen |
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| Přispěvatelé: | Immunology |
| Rok vydání: | 1993 |
| Předmět: |
Male
Adoptive cell transfer T-Lymphocytes Immunology Fluorescent Antibody Technique Bone Marrow Cells Thymus Gland Biology Immunotherapy Adoptive Immunophenotyping Mice Antigen Bone Marrow medicine Immunology and Allergy Animals Progenitor cell Cluster of differentiation Antibodies Monoclonal General Medicine Cell sorting Flow Cytometry Hematopoietic Stem Cells Molecular biology Rats Mice Inbred C57BL Haematopoiesis medicine.anatomical_structure Antigens Surface Female Bone marrow Stem cell Biomarkers |
| Zdroj: | International Immunology, 5(9), 1093-1098. Oxford University Press |
| ISSN: | 0953-8178 |
| Popis: | We searched for new cell surface markers that allow a positive identification of thymusrepopulating cells in the bone marrow (BM) of the mouse. Recently we raised two rat monocional antibodies (ER-MP12 and ER-MP20) that recognize cell surface antigens expressed by mouse haematopoietic progenitor cells, among which are progenitor cells of the macrophage lineage. Here we show that the ER-MP12 antigen, but not the ER-MP20 antigen, is also expressed by BM cells with thymus-repopulating ability. Using ER-MP12 and ER-MP20 in two-colour immunofluorescence analysis six subpopulatlons of BM cells can be identified. The thymusrepopulating ability of each BM subpopulation was assessed after fluorescence-activated cell sorting and subsequent intrathymic injection into sublethally irradiated Thy-1 congenic recipient mice. Thymus-repopulating activity appeared to be exclusively confined to two subsets of BM cells expressing either high or intermediate levels of the ER-MP12 antigen, but lacking ER-MP20 antigen expression. These BM subsets comprised 1-2% and 30% of total nucleated BM cells respectively. The frequency of thymus-repopulating cells was maximal in the minor BM subpopulation with the highest level of ER-MP12 antigen expression. We conclude that ER-MP12 detects a hitherto unknown cell surface marker expressed by BM cells with thymus-repopulating ability. |
| Databáze: | OpenAIRE |
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