Comparison of ELISA and TLC Methods for the Morphine Detection in Urine of Drug Abusers
| Autor: | Riahi Zanjani Bamdad, Sadeghi Mahmood, Lari Niloofar, Timcheh Hariri Alireza, Balali Mood Mahdi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
medicine.medical_specialty
Health Toxicology and Mutagenesis Drug abuser TLC Confirmation test Urine Toxicology 01 natural sciences 03 medical and health sciences 0302 clinical medicine lcsh:RA1190-1270 Medicine 030212 general & internal medicine Elisa method Confirmation Test lcsh:Toxicology. Poisons Chromatography Morphine business.industry Morphine derivatives 010401 analytical chemistry 0104 chemical sciences Surgery ELISA business medicine.drug |
| Zdroj: | Iranian Journal of Toxicology, Vol 10, Iss 3, Pp 47-50 (2016) |
| ISSN: | 2251-9459 2008-2967 |
| Popis: | Background: The current study was conducted to compare ELISA with thin layer chromatography (TLC) methods for diagnosis of morphine in the urine. Methods: Positive urine samples for morphine confirmed by immunochromatographic strips were collected from the Imam Reza Toxicology Laboratory, Mashhad University of Medical Sciences, Mashhad, Iran in 2012 for the current study. Then, the collated urine samples (70) were analyzed by both ELISA and TLC methods. Results: On analyzing samples by TLC, 57 out of 70 (81.4%) revealed morphine spot, whereas by ELISA method all samples were positive. The difference was statisticaly significant (P=0.0001). Both immunoassays had the same 100% positive results. The possible 18.6% false positive results might be due to drug interactions. TLC is more specific but time-consuming and less sensitive than ELISA is. However, TLC is an old method but more reliable than ELISA. Conclusion: Contrary to the claim that commercially available ELISA kits have a high specificity for detection of morphine derivatives; it seems that false positive results may occur. It is thus recommended that all positive results obtained from ELISA be checked by a cheap widely available confirmation test of TLC or ideally by a quantitative technique such as GC-Mass spectroscopy, particularly for legal purposes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|