Developing an in vivo toxicity assay for RNAi risk assessment in honey bees, Apis mellifera L
| Autor: | Jessica Jurzenski, Blair D. Siegfried, Marion D. Ellis, Natalie Matz, Ana María Vélez, Haichuan Wang, Xuguo Zhou |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Environmental Engineering Health Toxicology and Mutagenesis Biology medicine.disease_cause Risk Assessment Zea mays 01 natural sciences 03 medical and health sciences Honey Bees RNA interference Pollen Toxicity Tests Gene expression medicine Animals Environmental Chemistry Pest Control Biological RNA Double-Stranded Genetics Larva business.industry fungi Public Health Environmental and Occupational Health food and beverages Feeding Behavior General Medicine General Chemistry Honey bee Bees Plants Genetically Modified Pollution Biotechnology Coleoptera 010602 entomology RNA silencing 030104 developmental biology behavior and behavior mechanisms Insect Proteins Biological Assay RNA Interference Risk assessment business |
| Zdroj: | Chemosphere. 144:1083-1090 |
| ISSN: | 0045-6535 |
| DOI: | 10.1016/j.chemosphere.2015.09.068 |
| Popis: | Maize plants expressing dsRNA for the management of Diabrotica virgifera virgifera are likely to be commercially available by the end of this decade. Honey bees, Apis mellifera, can potentially be exposed to pollen from transformed maize expressing dsRNA. Consequently, evaluation of the biological impacts of RNAi in honey bees is a fundamental component for ecological risk assessment. The insecticidal activity of a known lethal dsRNA target for D. v. virgifera, the vATPase subunit A, was evaluated in larval and adult honey bees. Activity of both D. v. virgifera (Dvv)- and A. mellifera (Am)-specific dsRNA was tested by dietary exposure to dsRNA. Larval development, survival, adult eclosion, adult life span and relative gene expression were evaluated. The results of these tests indicated that Dvv vATPase-A dsRNA has limited effects on larval and adult honey bee survival. Importantly, no effects were observed upon exposure of Am vATPase-A dsRNA suggesting that the lack of response involves factors other than sequence specificity. The results from this study provide guidance for future RNAi risk analyses and for the development of a risk assessment framework that incorporates similar hazard assessments. |
| Databáze: | OpenAIRE |
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