Direct Comparison of Poly(ethylene glycol) and Phosphorylcholine Drug-Loaded Nanoparticles In Vitro and In Vivo
Autor: | Nicholas L. Fletcher, Kristofer J. Thurecht, Fan Chen, Dewan Taslima Akhter, Zachary H. Houston, Martina H. Stenzel, Janina-Miriam Noy |
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Rok vydání: | 2020 |
Předmět: |
Polymers and Plastics
Phosphorylcholine Nanoparticle Bioengineering 02 engineering and technology Conjugated system 010402 general chemistry 01 natural sciences Micelle Polyethylene Glycols Biomaterials chemistry.chemical_compound In vivo PEG ratio Materials Chemistry Tissue Distribution Particle Size Micelles 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry Drug delivery Biophysics Nanoparticles 0210 nano-technology Ethylene glycol |
Zdroj: | Biomacromolecules. 21(6) |
ISSN: | 1526-4602 |
Popis: | Phosphorylcholine is known to repel the absorption of proteins onto surfaces, which can prevent the formation of a protein corona on the surface of nanoparticles. This can influence the fate of nanoparticles used for drug delivery. This material could therefore serve as an alternative to poly(ethylene glycol) (PEG). Herein, the synthesis of different particles prepared by polymerization-induced self-assembly (PISA) coated with either poly(ethylene glycol) (PEG) or zwitterionic 2-methacryloyloxyethyl phosphorylcholine (MPC) and 4-(N-(S-penicillaminylacetyl)amino) phenylarsenonous acid (PENAO) was reported. The anticancer drug 4-(N-(S-penicillaminylacetyl)amino) phenylarsenonous acid (PENAO) was conjugated to the shell-forming block. Interactions of the different coated nanoparticles, which present comparable sizes and size distributions (76-85 nm, PDI = 0.067-0.094), with two-dimensional (2D) and three-dimensional (3D) cultured cells were studied, and their cytotoxicities, cellular uptakes, spheroid penetration, and cell localization profiles were analyzed. While only a minimal difference in behaviour was observed for nanoparticles assessed using in vitro experiment (with PEG-co- PENAO-coated micelles showing slightly higher cytotoxicity and better spheroid penetration and cell localization ability), the effect of the different physicochemical properties between nanoparticles had a more dramatic effect on in vivo biodistribution. After 1 h of injection, the majority of the MPC-co-PENAO-coated nanoparticles were found to accumulate in the liver, making this particle system unfeasible for future biological studies. |
Databáze: | OpenAIRE |
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